过表达N-Myc下游调节基因3促进Hela宫颈癌细胞增殖和迁移  

Overexpression of N-myc downstream regulated gene 3 promotes the proliferation and migration of Hela cervical cancer cells

作　　者：符婕[1] 白雪[2] 杨琳红 陈立平[1] 魏宏[1] 李晶[2] 齐亚灵[4] 王伟群[2] FU Jie, BAI Xue, YANG Lin-Hong,et al.(Department of Obstetrics and Gynecology of Jiamusi Central Hospital, Jiamusi154002, Heilongjiang, Chin)

机构地区：[1]佳木斯市中心医院妇产科,黑龙江佳木斯154002 [2]佳木斯大学基础医学院 [3]佳木斯大学附属第一医院 [4]海南医学院

出　　处：《中国老年学杂志》2018年第6期1436-1438,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金面上项目(No.81272854);黑龙江省自然科学基金项目(No.D200966);海南医学院引进人才科研启动基金项目(No.2017002)

摘　　要：目的研究过表达N-Myc下游调节基因(NDRG)3对Hela宫颈癌细胞增殖和迁移的作用。方法构建NDRG3过表达载体,利用Lipofectamine 2000转染试剂建立稳定转染NDRG3的Hela宫颈癌细胞株,细胞计数试剂(CCK)8和Transwell方法研究细胞增殖和迁移能力,酶联免疫吸附实验研究趋化因子CXC配体(L)5的表达情况。结果过表达NDRG3可明显促进Hela的增殖和迁移能力,并上调Hela的分泌性CXCL5蛋白水平。结论 NDRG3可经上调肿瘤相关基因CXCL5的水平参与宫颈癌进程。Objective To study the effects of N-myc downstream regulated gene （NDRG）3 overexpression on the proliferation and migration of Hela cervical cancer cells. Methods After building NDRG3 expression vector, Lipofectamine 2000 transfection reagent was used to establish a stable transfection NDRG3 Hela cervical cancer cell lines, cell proliferation and migration ability were detected using CCK8 and Transwell methods, enzyme-linked immunosorbent assay was utilized to test the expression of CXC chemokine ligand （CXCL）5. Results Overexpression of NDRG3 significantly promoted the proliferation and migration of Hela, and up-regulated the secretion level of CXCL5 of Hela. Conclusions NDRG3 can participate in the cervical cancer process by up-regulating tumor-associated gene CXCL5 level.

关 键 词：宫颈癌 N-Myc下游调节基因3 趋化因子CXC配体5 

分 类 号：R737.3[医药卫生—肿瘤]