触珠蛋白作为胆道肿瘤胆汁和血清生物标志物的研究  

Application value of haptoglobin as bile and serum biomarkers for biliary tract carcinoma

作　　者：郑晓[1] 吴叶晨[1] 高道键[1] 胡冰[1] Zheng Xiao, Wu Yechen, Gao Daojian, Hu Bing.(Department of Endoscopy, Eastern Hepatobiliary Hospital, the Second Military Medical University, Shanghai 200438, Chin)

机构地区：[1]第二军医大学附属东方肝胆外科医院内镜科,上海200438

出　　处：《中华消化内镜杂志》2018年第3期171-174,共4页Chinese Journal of Digestive Endoscopy

基　　金：上海市医学领军人才计划（2015-83）

摘　　要：目的探寻胆道肿瘤潜在的生物标志物，为胆道肿瘤的早期诊断提供新方向。方法收集经病理结果证实的恶性胆道肿瘤和良性胆道疾病患者的胆汁、血清标本，应用二维液相色谱串联质谱分析（2D-LC-MS/MS）技术检测良、恶性胆道病变患者胆汁之间的差异表达蛋白，并应用蛋白免疫印迹法对胆汁及血流中差异表达蛋白进行验证。结果4组蛋白标本检测出10个差异表达蛋白，其中触珠蛋白在胆道肿瘤组的表达明显上调。免疫印迹法验证其在恶性肿瘤组胆汁和血清中高表达。 结论触珠蛋白在胆道恶性肿瘤中表达上调，可能为胆道肿瘤潜在的生物标志物。ObjectiveTo detect cancer-related biomarkers for early diagnosis of biliary tract carcinoma.MethodsBile and serums were obtained from patients with histopathologically proven malignant or benign biliary diseases, and clinical data of patients were recorded. Two-dimensional liquid chromatography tandem mass spectrometry （2D-LC-MS/MS） was applied to detect the differential protein profiles in the bile. Western blot was used to validate the results in the biles and serums.ResultsTen differential protein profiles were detected in 4 protein groups. Among them, the expression of haptoglobin significantly increased in the bile of patients with malignancy. This result was further verified using Western blot.ConclusionHaptoglobin may be a potential biomarker for biliary tract carcinoma.

关 键 词：胆道肿瘤 触珠蛋白 生物学标记 蛋白组学 串联质谱法 液相色谱 

分 类 号：R735.8[医药卫生—肿瘤]