高效液相色谱测定诺丽果实中有机酸含量的方法  

Determination of Organic Acids Content in Noni by HLPC

作　　者：李玖慧[1] 吴晓鹏[1] 袁靖喆 马子龙[1] LI Jiuhui ,WU Xiaopeng ,YUAN Jingzhe, MA Zilong(Institute of Tropical Bioscience and Bioteehnology / Ministry of Agriculture Key Laboratory of Biology Resources of Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan and Genetic 57110)

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带农业工程》2017年第5期48-51,共4页Tropical Agricultural Engineering

摘　　要：采用高效液相色谱法测定诺丽果实中草酸、酒石酸、苹果酸、抗坏血酸和柠檬酸的含量。样品用磷酸二氢钾+磷酸水溶液经超声波提取,用SinoChrom ODS-BP色谱柱分离,流动相为磷酸二氢钾+磷酸溶液pH2.8,流速0.6 m L/mi n。在该条件下中草酸、酒石酸、苹果酸、抗坏血酸和柠檬酸得到很好的分离,分别在1.0-2.5、1.0-2.5、1.5-2.5和1.0-2.5μg的范围内有良好的线性范围,相关系数R^2分别为0.999 0、1.000 0、0.999 9、0.999 9和1.000 0,最低检出限分别为1.0、2.0、2.5、1.5和3 mg/kg,加标回收率分别为101.3%、98.7%、97.3%、98.3%和97.5%。该方法快速、准确,能用于测定诺丽果实中有机酸的含量。Organic acid in Noni sample were determined by HPLC. Samples was extracted by phos- phate buffer solution and ultrasonic, separated on the SinoChrom ODS-BP column using phosphate buffer solution with pH 2.8 as the mobile phase, and the flow rate of 0.6ml/min. In these condition, oxalic acid, tartaric acid, malic acid, ascorbic acid and citric acid could be well separated. Organic acid showed a good linearity in 1.0-2.5, 1.0-2.5, 1.5-2.5 and 1.0-2.5 μg（R2=0.999 0, 1.000 0, 0.999 9, 0.9999, 1.0000）, the LOD were 1.0, 2.0, 2.5, 1.5 and 3 mg/kg and the recoveries were 101.3%, 98.7%, 97.3 %, 98.3 % and 97.5 %. The verified results demonstrated that this method was precise and accu- rate and could be used for determination of organic acids in noni.

关 键 词：有机酸 高效液相色谱 诺丽 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]