Tipα与PTEN在人胃癌组织中的表达及意义  

The expression and significance of Tipα and PTEN in gastric cancer

作　　者：杨铭 刘小媚 胡凌佼 范小青 Yang Ming, Liu Xiaomei, Hu LingJiao, Fan Xiaoqing(Department of Gastroenterology, The First People＇S Hospital of Yongzhou, Yongzhou 425100, China ; Department of Gastroenterology, The Central Hospital of Yongzhou, Yongzhou 425100, China ; Yongzhou Clinical College, University of South China, Yongzhou 425100, China)

机构地区：[1]湖南省永州市第一人民医院消化内科,425100 [2]湖南省永州市中心医院消化内科,425100 [3]南华大学永州临床学院,湖南省永州425100

出　　处：《中国医师杂志》2018年第3期371-373,共3页Journal of Chinese Physician

基　　金：2016年度湖南省教育厅科学研究项目(16C1616)~~

摘　　要：目的探讨TiPα、PTEN与胃癌的相关性。方法 RT-qPCR技术检测胃癌、癌旁组织、正常胃组织中肿瘤坏死因子α诱导蛋白(TiPα)、第10号染色体丢失的磷酸酶基因(PTEN)相对定量。结果 (1)Tipα、PTEN在胃癌、癌旁组织、正常胃组织中的表达量分别为0.83±0.07、0.16±0.10、0.10±0.12及0.23±0.05、0.92±0.07、1.84±0.13;胃癌与癌旁以及正常胃黏膜组织相比较,差异均有统计学意义(P<0.05)。(2)TiPα、PTEN在低、高分化胃癌组织中表达量分别为1.10±0.04、0.36±0.05及0.08±0.05、0.36±0.04,二者表达量与胃癌的分化程度有关(P<0.05);Tipα、PTEN在有、无淋巴结转移胃癌组织中表达量分别为0.18±0.12、0.82±0.09及0.10±0.12、0.38±0.10,二者表达量与胃癌有无淋巴结转移有关(P<0.05);PTEN在胃癌TNMⅠ～Ⅱ期、Ⅲ～Ⅳ期表达量为0.25±0.04、0.06±0.07,PTEN表达量与胃癌TNM分期有关(P<0.05)。(3)Tipα与PTEN在胃癌组织中的表达呈负相关(r=-0.883,P<0.05)。结论 (1)Tipα可能为促癌因子;PTEN为抑癌因子;(2)胃癌中Tipα与PTEN表达存在负相关。Objective To investigate the correlation between tumor necrosis factor -α inducing protein （Tipα）, phosphatase and tensin homologue deleted onchromosome ten （PTEN） and gastric cancer. Methods To detecte the relative quantification of Tipα and lost phosphatase gene of tenth chromosome PTEN in gastric cancer, paracancerous tissues and normal gastric tissues by real-time quantitative polymerase chain reaction （RT-qPCR） technique. Results （1） The expression levels of Tipα and PTEN in gastric cancer, paracancerous tissues and normal gastric tissues were 0. ±0. 07, 0. 16 ±0. 10, 0. 10 ±0. 12, 0. 23 ±0. 05, 0. 92 ±0.07, 1.84 ±0. 13,respectively. Comparison of gastric cancer with paracancerous tissues and normal gastric tissue, there were significant statistical differences （ P 〈 0. 05 ）. （2） The expression levels of Tipct and PTEN in low and well differentiated gastric carcinoma werel. 10 ±0. 04, 0. 36 ±0.05, 0. 08 ±0. 05, 0. 36 ±0. 04, respectively, both of which are significantly associated with the degree of differentiation of gastric cancer（ P 〈 0. 05 ） ;The expression levels of Tipα and PTEN in gastric cancer tissues with or without lymph node metastasis were 0. 18 ±0. 12, 0. 82 ±0. 09, 0. 10 ±0. 12, 0. 38 ±0. 10,both had significant correlation with lymph node metastasis of gastric cancer（ P 〈 0. 05 ） ;The expression of PTEN in gastric cancer tumor node metastasis （ TNM ） Ⅰ - Ⅱ and Ⅲ -Ⅳ were 0. 25 ±0. 04, 0. 06± 0. 07, which means PTEN was significantly correlated with gastric cancer TNM staging（P 〈 0. 05 ）. （3） The expressions of Tipα and PTEN were negatively correlated in gastric cancer tissues （ r = - 0. 883 ,P 〈 0. 05 ）. Conclusions （1） Tipα may be a cancer-promoting factor;PTEN maybe a tumor suppressor factor;（2） There is a negative correlation between the expression of Tipα and PTEN in gastric carcinoma.

关 键 词：胃肿瘤/病理学/代谢 肿瘤坏死因子仅诱导蛋白/代谢 PTEN磷酸水解酶/代谢 

分 类 号：R735.2[医药卫生—肿瘤]