抑菌浓度米诺环素对成骨细胞增殖、分化和矿化的影响  

Effects of inhibitory concentration minocycline on the proliferation,differentiation,and mineralization of osteoblasts

作　　者：邵华英 张一弓[1,2,3] 杨雪 张琼月 吴小红 Shao Huaying1,2,3, Zhang Yigong1,2,3, Yang Xue1,2,3, Zhang Qiongyue1,2,3, Wu Xiaohong1,3(1. Dept. of Prosthodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China; 2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China; 3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, Chin)

机构地区：[1]重庆医科大学附属口腔医院修复科 [2]口腔疾病与生物医学重庆市重点实验室 [3]重庆市高校市级口腔生物医学工程重点实验室,重庆401147

出　　处：《华西口腔医学杂志》2018年第2期140-145,共6页West China Journal of Stomatology

基　　金：国家自然科学基金(81200767;H1402);重庆市基础科学与前沿技术研究专项资助(cstc2017jcyj AX0215);重庆市渝中区科技计划项目(20170117);重庆市渝北区科技计划项目(渝北财签[2011]102号);2016年重庆高校创新团队建设计划资助项目(CXTDG201602006)

摘　　要：目的研究抑菌浓度的米诺环素对成骨细胞增殖、分化和矿化及Runt相关转录因子2(Runx2)、碱性磷酸酶(ALP)、骨桥蛋白(OPN)mRNA表达的影响。方法将米诺环素溶液(0、0.1、0.5、1、10μg·mL^(-1))与原代成骨细胞共培养,CCK-8检测增殖活性,通过ALP活性检测、茜素红染色、荧光定量聚合酶链反应检测探讨米诺环素对成骨细胞分化、矿化的影响。结果 0.1、0.5、1μg·mL^(-1)的米诺环素可以促进细胞的增殖,上调ALP、Runx2mRNA的表达水平,增加钙含量及钙化结节的形成,其中1μg·mL^(-1)具有最大促进作用(P<0.05);当浓度为10μg·mL^(-1)时这种促进作用开始下降,并对ALP活性和OPN表达有显著抑制作用(P<0.01)。结论适宜抑菌浓度的米诺环素能促进成骨细胞增殖,上调Runx2、ALP、OPN的表达水平,促进成骨细胞分化和矿化。Objective To study the effect of the inhibitory concentration minocycline on the proliferation, differentiation, and expression of Runt-related transcription factor 2 （Runx2）, alkaline phosphatase （ALP） and osteopontin （OPN） mRNA of osteoblasts. Methods Primary osteoblasts were cultured in osteogenic induction medium containing 0, 0.1, 0.5, 1, 10 μg·mL^-1 minocycline. Cell counting kit-8 was used to observe cell proliferation. ALP activity assay, alizarin red S staining, and real-time quantitative polymerase chain reaction （PCR） were used to determine cell differentiation and mineralization. Results The groups with 0.1, 0.5, 1 μg·mL^-1 minocycline promoted cell proliferation. The mRNA expression levels of ALP and Runx2 were up-regulated. Osteoblast-mediated mineralization was increased. The group with 1 μg·mL^-1 showed maximal promotion effect （P〈0.05）. When the concentration increased to 10 μg·mL^-1, the promoting effect began to decline, and the ALP activity and OPN expression were significantly inhibited （P〈0.01）. Conclusion Appropriate concentration of minocycline can promote osteoblasts proliferation, up-regulate the expression levels of Runx2, ALP and OPN, and increase the differen-tiation and mineralization of osteoblasts.

关 键 词：米诺环素 成骨细胞 碱性磷酸酶 增殖 

分 类 号：R781.05[医药卫生—口腔医学]