化瘀祛痰方对AS家兔肝脏线粒体呼吸链酶复合物的影响  

Effect of Huayu Qutan Recipe on Respiratory Chain Enzyme Complexes of Liver Mitochondria in AS Rabbits

作　　者：陈宁[1] 宋囡[1] 贾连群[1] 陈丝 王健[1] 王群[1] 杨关林[1] CHEN Ning, SONG Nan, JIA Lian-qun , CHEN Si, WANG Jian, WANG Qun, YANG Guan-lin(Translational Medicine Research Center of Traditional Chinese Medicine （TCM） in Liaoning Province, Liaoning University of TCM , Shenyang 110847, China)

机构地区：[1]辽宁中医药大学辽宁省中医转化医学研究中心

出　　处：《中国实验方剂学杂志》2018年第7期171-176,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81774022);国家百千万人才工程项目(2017-58);辽宁省高等学校优秀人才支持计划项目(LR2015041)

摘　　要：目的：探究化瘀祛痰方对动脉粥样硬化（atherosclerosis，AS）家兔肝脏线粒体呼吸链酶复合物的影响，并探讨其相关的作用机制。方法：健康SPF级雄性新西兰白兔90只，随机分为正常组、模型组、化瘀祛痰方高、中、低剂量组（4，8，16g·kg^-1），辛伐他汀组（1．4mg·kg^-1），每组15只。正常组给予基础饲料喂养，其他组采用高脂饲料喂养，建立动脉粥样硬化模型，造模8周后，化瘀祛痰方高、中、低剂量组与辛伐他汀组给予相应药物溶液，正常组和模型组给予等量生理盐水，4周后，全自动生物化学分析仪检测血清甘油三酯（triglyceride，TC），胆固醇（cholesterol，TC），高密度脂蛋白（high-density lipoprotein cholesterol，HDL-C），低密度脂蛋白（low-density lipoprotein cholesterol，LDL-C）的含量；苏木素-伊红（HE）和油红O染色观察肝组织形态学改变；分光光度计法检测家兔肝脏线粒体呼吸链酶复合物活性；Blue-Native-Page技术检测线粒体呼吸链酶复合物含量。结果：与正常组比较，模型组家兔血清中TC，TG，LDL．C显著升高，HDL．C显著降低；与模型组比较，化瘀祛痰方高、中、低剂量组与辛伐他汀组家兔血清中TC，TG，LDL-C显著降低，HDL-C显著升高（P〈0．05，P〈0．01），其中化瘀祛痰方中以高剂量效果最为显著。HE及油红0染色结果显示模型组较正常组比较家兔肝组织肿胀明显、脂肪空泡清晰可见，胞浆内脂滴蓄积明显；与模型组比较，化瘀祛痰高、中、低剂量组及辛伐他丁组脂肪空泡明显减少或消失，肝组织形态恢复或部分恢复正常，其中化瘀祛痰方中以高剂量效果最为显著。与正常组比较，模型组肝脏线粒体呼吸链酶复合物I，Ⅱ，Ⅲ，Ⅳ，V活性及含量显著降低（P〈0．01）；与模型组比较，化瘀祛痰方高剂量组与辛伐他汀组肝脏线粒体呼吸链酶复合物I，Objective： To study the effects of Huayu Qutan recipe on liver mitochondrial respiratory chain enzyme complexes in atherosclerosis （AS） rabbits. Method： Totally 90 healthy male SPF New Zealand white rabbits were randomly divided into the normal group, the model group, high, middle and low dose Huayu Qutan recipe groups （4, 8, 16 g·kg-1 ） , simvastatin group （ 1.4 mg.kg-1 ） , 15 rats in each group. The normal group was fed with basic feeding, and other groups were fed with high-fat diet to establish the atherosclerosis model. After 8 weeks, high, middle and low dose Huayu Qutan recipe groups and simvastatin group were given with corresponding drug solution, while the normal group and model group were given with the same amount of saline. After 4 weeks, the levels of triglyceride （TG） , cholesterol （TC） , low-density lipoprotein cholesterolc （LDL-C） and high-density lipoprotein cholesterol （HDL-C） in serum were tested by the automatic biochemistry analyzer. Hematoxylin-eosin （HE） staining and oil red O staining were used to observe the changes of liver tissue morphology. The activity of liver mitochondrial respiratory chain enzyme complexes was tested by the spectrophotometry. The content of mitochondrial respiratory chain enzyme complexes was tested by Blue-Native- Page. Result： As compared with the normal group, TC, TG, LDL-C levels in serum were increased significantly, and HDL-C level was decreased significantly in model group. As compared with the model group, TC, TG, LDL-C levels were decreased significantly, and HDL-C was increased significantly in high, middle and low dose Huayu Qntan recipe groups and simvastatin group （P 〈 0.01 ）. The effect of high dose Huayu Qutan recipe was most obvious. HE and oil red O staining results showed that as compared with the normal group, the swelling of rabbit liver tissues was obvious and fat vacuoles were clearly visible, with significantly accumulated lipid droplets in the cytoplasm in model group. As compared with the model

关 键 词：化瘀祛痰方 动脉粥样硬化 线粒体呼吸链酶复合物 脂质沉积 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]