肺炎支原体大环内酯类耐药基因突变与基因分型的相关性  

Relationship between macrolide resistance mutations in Mycoplasma pneumoniae and its genotype

作　　者：闫超[1] 薛冠华 孙红姝[1] 李少丽[1] 赵汉青[1] 冯燕玲[1] Yan Chao, Xue G uanhua, Sun Hongmei, Li Shaoli, Zhao Hanqing, Feng Yanling(Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, Chin)

机构地区：[1]首都儿科研究所细菌研究室,100020

出　　处：《中华微生物学和免疫学杂志》2018年第1期31-36,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81672026）;北京市自然科学基金（7152025）

摘　　要：目的分析肺炎支原体23S rRNA中大环内酯类耐药基因突变与MLVA基因分型之间的相关性。方法利用套式PCR法检测143例肺炎支原体（Mycoplasma pneumoniae, Mp）阳性标本（北京54例，美国59例，澳大利亚30例）的23S rRNA中大环内酯类耐药相关基因突变，并利用单管多重PCR结合毛细管电泳的方法对这些标本进行多位点可变数目串联重复序列分析（MLVA分型），分析二者的相关性。结果143例标本分为10个MLVA基因型。54例来自北京的Mp阳性标本分为4个MLVA基因型，主要基因型是M4-5-7-2（44/54，81.5%）和M3-5-6-2（7/54，13.0%）；59例美国标本可分为6个MLVA基因型，主要基因型是M4-5-7-2 （27/59, 45.8%），M3-5-6-2（18/59，30.5%）和M3-6-6-2（11/59，18.6%）；30例澳大利亚悉尼标本可分为5个MLVA基因型，主要基因型是M3-5-6-2（12/30，40.0%），M4-5-7-2（10/30，33.3%）和M3-5-7-2（5/30，16.7%）。143例标本中57例（北京49例，美国7例，悉尼1例）检出耐药基因突变，其中50例（87.7%）标本的MLVA分型为M4-5-7-2。结论肺炎支原体的耐药基因突变与MLVA分型的M4-5-7-2型之间存在显著相关性。ObjectiveTo analyze the relationship between macrolide resistance mutations in Mycoplasma pneumoniae （Mp） and its genotype by multiple-locus variable-number tandem-repeat analysis （MLVA）.MethodsOne hundred and forty-three Mp-positive specimens were collected in Beijing （54 collected at the Affiliated Children′s Hospital of the Capital Institute of Pediatrics）, the United States （59 collected at four different geographical locations： Kansas City, Missouri; Seattle, Washington; New York, New York; Chicago, Illinois） and Australia （30 provided by the diagnostic laboratory at the Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Sydney）. Nested PCR was used to detect mutations in 23S rRNA. A capillary electrophoresis-based single tube multiplex PCR （mPCR-CE） was used to analyze the MLVA types of Mp in those samples.ResultsA2063G mutation was identified in 57 specimens including 49 from Beijing, seven from the United States and one from Australia. The 143 Mp-positive specimens were typed into 10 distinct MLVA types. Fifty-four specimens collected in Beijing belonged to four MLVA types, which were M4-5-7-2 （44/54, 81.5%）, M3-5-6-2 （7/54, 13.0%）, M4-5-6-2 （2/54, 3.70%） and M4-5-5-2 （1/54, 1.85%）. Fifty-nine specimens collected in the United States belonged to six MLVA types including M4-5-7-2 （27/59, 45.8%）, M3-5-6-2 （18/59, 30.5%）, M3-6-6-2 （11/59, 18.6%）, M3-5-6-1 （1/59, 1.69%）, M4-5-7-3 （1/59, 1.69%） and M5-5-7-2 （1/59, 1.69%）. Thirty specimens of Mp from Australia were grouped to five types with M3-5-6-2 （12/30, 40.0%） and M4-5-7-2 （10/30, 33.3%） and M3-5-7-2 （5/30, 16.7%） being the predominant types. Macrolide resistance mutations were detected in 57 out of 143 specimens （49 from Beijing, seven from the United States and one from Sydney） and 50 of them were MLVA type of M4-5-7-2.ConclusionThe MLVA type of M4-5-7-2 is associated with macrolide

关 键 词：肺炎支原体 大环内酯类耐药 23S RRNA MLVA分型 

分 类 号：R446.5[医药卫生—诊断学]