粪菌移植与5-氨基水杨酸在小鼠实验性结肠炎中的疗效观察  

Efficacy of fecal microbiota transplantation and 5-aminosalicylic acid in management of experimental colitis in mice

作　　者：曾翠 余红璐 陈正莲 杨歆睿[2] 熊枝繁 Cui Zeng, Hong-Lu Yu, Zheng-Lian Chen, Xin-Rui Yang, Zhi-Fan Xiong(Cui Zeng, Hong-Lu Yu, Zhi-Fan Xiong, Department of Gastroenterology, Liyuan Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430077, Hubei Province, China ;Zheng-Lian Chen, Xin-Rui Yang, Department of Pathology, Liyuan Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430077, Hubei Province, Chin)

机构地区：[1]华中科技大学同济医学院附属梨园医院消化内科,湖北省武汉市430077 [2]华中科技大学同济医学院附属梨园医院病理科,湖北省武汉市430077

出　　处：《世界华人消化杂志》2018年第7期411-417,共7页World Chinese Journal of Digestology

摘　　要：目的观察粪菌移植(fecal microbiota transplantation,FMT)与5-氨基水杨酸(5-aminosalicylic acid,5-ASA)在小鼠实验性结肠炎模型中的疗效.方法将40只6周龄左右的♂C57BL/6小鼠,随机分为2组,正常组(8只)和模型组(32只),适应性喂养2 wk后,模型组连续自由饮用3%葡聚糖硫酸钠(dextran sulfate sodium,DSS)5 d,建立实验性结肠炎模型,于第6天更换为纯净水,A组自由饮用纯净水,适应性喂养3 d后,将模型组随机分为4组,每组8只,分别为:模型对照组、FMT组、5-ASA组、联合组(FMT+5-ASA),FMT组、5-ASA组及联合组于第9、11、13天粪便给予粪菌滤液、5-ASA灌肠液及粪菌滤液与5-ASA的混合液灌肠,其余两组给予生理盐水灌肠.每日进行小鼠的疾病活动指数评分(disease active index,DAI),于第14天处死小鼠,采集血液标本行IL-10检测,测量结肠长度,留取结肠组织行HE染色,并评估组织损伤指数(histological index,HI).结果与模型组相比,5-ASA组与联合组均取得较好的疗效,DAI评分、HI评分较治疗前明显降低,差异有统计学意义(P<0.05),但仍高于空白对照组(P<0.05),F M T组的D A I评分、HI评分较前有治疗前有所缓解,但其与模型对照组差异无统计学意义(P<0.05);5-ASA组与联合组的I L-10水平高于模型对照组(P<0.05),而FMT组的IL-10水平较模型对照组增加,但差异无统计学意义(P>0.05).结论 FMT在小鼠结肠炎模型中确实有一定疗效,但仍不如氨基水杨酸制剂,其与氨基水杨酸制剂的联合使用也并没有取得更好的效果,可能与治疗方式、治疗周期等因素有关.AIM To evaluate the efficacy of fecal microbiota transplantation （FMT） and 5-aminosalicylic acid （5-ASA） in the treatment of experimental colitis in a mouse model.METHODS Forty 6-wk-old male C57BL/6 mice were randomly divided into either a normal group （8 rats） or a model group （32 rats）. After 2 wk of feeding, the model group was continuously treated with 3% dextran sulfate sodium （DSS） for 5 d to establish an experimental colitis model. After adaptive feeding for 3 d, the model group was further randomly divided into four groups （8 rats in each group）： a model control group, an FMT group, a 5-ASA group, and a combination group （FMT ＋ 5-ASA）. The FMT group, 5-ASA group, and combination group were given fecal filtrate, 5-ASA enema solution, and fecal filtrate plus 5-ASA enema solution on the 9th, 11th, and 13th days, respectively. The other two groups were given physiological salt solution enema. Mice were sacrificed on the 14th day, and blood samples were collected for interleukin-10 （IL-10） detection. Colon length was measured, and colon tissues were harvested for hematoxylin and eosin （HE） staining to evaluate histological index （HI）. RESL TS Compared with the model group, the 5-ASA group and the combination group achieved better curative score in the two groups were significantly lower than those before treatment （P 〈 0.05）, but still higher than those in the normal control group （P 〈 0.05）. The DAI score and HI score in the FMT group were improved compared with those before treatment, but there was no significant difference between the FMT group and the model control group （P 〉 0.05）. The level of IL-10 in the FMT group was higher than that in the model control group （P 〈 0.05）, but the difference was not statistically significant （P 〉 0.05）.

关 键 词：溃疡性结肠炎 粪菌移植 氨基水杨酸 肠道菌群 免疫机制 

分 类 号：R574.62[医药卫生—消化系统]