同位素特异肽段在乳清蛋白定量分析中的应用  

Application of isotope specific peptides in quantitative analysis of whey protein

作　　者：王震 姚美伊[2] 凌云[2] 李晓娟[2] 李奇峰[1] 陈达[1] WANG Zhenl＇2,YAO Meiyi2,LING Yun2,LI Xiaojuan2,LI Qifeng1,CHEN Dal(1.TianJin University, Tianjin, 300071,China; 2. Chinese Academy of Inspection and Quarantine, Beijing 100176, Chia)

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300071 [2]中国检验检疫科学研究院,北京100176

出　　处：《中国乳品工业》2018年第3期41-44,共4页China Dairy Industry

基　　金：北京市自然科学基金北京三元食品股份有限公司联合资助项目(S150008);国家自然科学基金(21305101;61378048)

摘　　要：采用液相色谱串联质谱法,以同位素特异肽段作为内标物,对婴幼儿配方奶粉中的α-乳白蛋白和β-乳球蛋白进行了定量分析。结果表明,方法的线性关系良好,实验室内重复性为α-乳白蛋白的日内相对标准偏差(RSD)在1.23%~10.1%之间,日间RSD在4.13%~12.2%;β-乳球蛋白的日内RSD在1.06%~10.3%之间,日间RSD在1.52%~12.0%之间。实验室间的重现性为α-乳白蛋白的RSD在10.4%~18.4%之间;β-乳球蛋白的RSD在10.7%~23.6%之间。表明此方法可准确测定婴幼儿奶粉中的α-乳白蛋白及β-乳球蛋白的质量分数,但实验室间的稳定性需要进一步考察。The study quantified the total α-la and β-lg in infant formula with liquid chromatography tandem mass spectrometry and using isotope specific peptides as internal standard substance. Results showed that the method had a good linear relationship. For α-la, Relative standard deviation （RSD） of intraday precision was between 1.23%- 10.1% and RSD of inter-day precision was between 4.13% - 12.2% ; For β-lg, RSD of intraday precision is between 1.06% - 10.3% and RSD of inter-day precision was 4.13% - 12.2% . For repeatability of laboratory, RSD of α-la was between 10.4% - 18.4% and RSD ofβ-lg was between 10.7% - 23.6%. Results indicated that this method can be used to determinethe content of α-la and β-lg in infant formula, but the stabihty between laboratory needed further investigation.

关 键 词：高效液相串联质谱 同位素特异肽 乳清蛋白 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]