Enrichments of methanotrophic–heterotrophic cultures with high poly-β-hydroxybutyrate(PHB) accumulation capacities  

作　　者：Tingting Zhang Xiaowei Wang Jiti Zhou Yu Zhang 

机构地区：[1]Key Laboratory of Industrial Ecology and Environmental Engineering, School of Environmental Science and Technology, Dalian University of Technology

出　　处：《Journal of Environmental Sciences》2018年第3期133-143,共11页环境科学学报（英文版）

基　　金：supported by the National Key Scientific and Technology Project for Water Pollution Treatment of China (No.2012ZX07202006)

摘　　要：Methanotrophic–heterotrophic communities were selectively enriched from sewage sludge to obtain a mixed culture with high levels of poly-β-hydroxybutyrate（PHB）accumulation capacity from methane.Methane was used as the carbon source,N2as sole nitrogen source,and oxygen and Cu content were varied.Copper proved essential for PHB synthesis.All cultures enriched with Cu could accumulate high content of PHB（43.2%–45.9%）,while only small amounts of PHB were accumulated by cultures enriched without Cu（11.9%–17.5%）.Batch assays revealed that communities grown with Cu and a higher O2content synthesized more PHB,which had a wider optimal CH4：O2range and produced a high PHB content（48.7%）even though in the presence of N2.In all methanotrophic–heterotrophic communities,both methanotrophic and heterotrophic populations showed the ability to accumulate PHB.Although methane was added as the sole carbon source,heterotrophs dominated with abundances between 77.2%and 85.6%.All methanotrophs detected belonged to type II genera,which formed stable communities with heterotrophs of different PHB production capacities.Methanotrophic–heterotrophic communities were selectively enriched from sewage sludge to obtain a mixed culture with high levels of poly-β-hydroxybutyrate（PHB）accumulation capacity from methane.Methane was used as the carbon source,N2as sole nitrogen source,and oxygen and Cu content were varied.Copper proved essential for PHB synthesis.All cultures enriched with Cu could accumulate high content of PHB（43.2%–45.9%）,while only small amounts of PHB were accumulated by cultures enriched without Cu（11.9%–17.5%）.Batch assays revealed that communities grown with Cu and a higher O2content synthesized more PHB,which had a wider optimal CH4：O2range and produced a high PHB content（48.7%）even though in the presence of N2.In all methanotrophic–heterotrophic communities,both methanotrophic and heterotrophic populations showed the ability to accumulate PHB.Although methane was added as the sole carbon source,heterotrophs dominated with abundances between 77.2%and 85.6%.All methanotrophs detected belonged to type II genera,which formed stable communities with heterotrophs of different PHB production capacities.

关 键 词：METHANOTROPH Activated sludge PHB N2-fixation Methane monooxygenase 

分 类 号：X172[环境科学与工程—环境科学] X703