Ghrelin、GHRP-6以及胃动素对大鼠胃肠运动影响研究  

The Effects of Ghrelin, GHRP-6 and Motilin on the Gastroprokinetic in Rats

作　　者：王玮[1,2] 栾晓 柳洋[3] 郭菲菲 孙向荣[1] 徐珞[1] WANG Wei1,2, LUAN Xiao1, LIU Yang3, GUO Fei-fei1, SUN Xiang-rong1, XU Luo1(1 Dept. of Pathophysiology, Medical College of Qingdao University, Qingdao, Shandong, 266021, China; 2 Qilu Medical University, Zibo, Shandong, 255213, China; 3 Qingdao NO.9 people＇s hospital, Qingdao, Shandong, 266000, Chin)

机构地区：[1]青岛大学医学院病理生理学教研室,山东青岛266021 [2]淄博齐鲁医药学院,山东淄博255213 [3]青岛市第九人民医院,山东青岛266000

出　　处：《现代生物医学进展》2018年第3期428-432,452,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81470815;81270460;81500414);山东省优秀中青年科学基金项目(BS2014YY009);青岛市科技局项目(14-2-3-3-nsh)

摘　　要：目的:探究ghrelin、GHRP-6以及胃动素对大鼠胃肠运动的影响。方法:在体实验观察ghrelin、GHRP-6及胃动素对大鼠胃排空及小肠推进的影响,离体实验观察ghrelin、GHRP-6及胃动素对大鼠电场刺激引起胃窦胃底平滑肌舒缩反应的影响,RT-PCR观察ghrelin mRNA在胃的表达。结果:在体研究发现ghrelin及GHRP-6能够促进胃排空及小肠推进,胃动素对胃排空及小肠推进无影响。离体研究发现,ghrelin 1μM能够抑制胃底平滑肌4-8Hz开电刺激下的舒张反应,并增强胃底平滑肌1-8Hz及胃窦平滑肌4-16Hz断电刺激下的收缩反应;GHRP-6 1μM能够抑制胃底平滑肌4-8Hz开电刺激下的舒张反应,并增强胃底平滑肌1-2Hz、8-16Hz及胃窦平滑肌4-8Hz断电刺激下的收缩反应;L-NAME增强ghrelin、GHRP-6诱导的胃底和胃窦平滑肌条舒缩作用。大鼠胃窦及胃底分布有ghrelin受体mRNA。结论:ghrelin和GHRP-6能够加快胃排空促进小肠推进,该效应可能是通过迷走神经通路及胆碱能兴奋产生的。Objective： We compared the gastroprokinetic effects of ghrelin, the ghrelin receptor agonist, growth hormone releasing peptide 6（GHRP-6）, and motilin in rats in vivo and in vitro. Methods： In vitro observation the effect of ghrelin, GHRP-6 and motilin on rat gastric emptying and small intestinal propulsion, in vitro experimental observation the effect of ghrelin, GHRP-6 and motilin on the fundic or antral strips responses to EFS, RT-PCR observation the expression of ghrelin mRNA in the stomach. Results： Ghrelin and GHRP-6 but not motilin accelerated gastric emptying and transit in rats. 1 μM ghrelin can inhibit the on-relaxation of fundic strips（4-8 Hz）, and enhance off-response of fundic（1-8 Hz） and antral strips（4-16 Hz）; 1 μM GHRP-6 can inhibit the on-relaxation of fundic strips（4-8 Hz）, and enhance off-response of fundic（1-2,8-16 Hz） and antral strips（4-8 Hz）; the effect of ghrelin and GHRP-6 on fundic and antral strips can be enhance by L-NAME. The fundic and antral can express ghrelin mRNA. Conclusion： Ghrelin and GHRP-6 but not motilin accelerate gastric emptying and transit by activating cholinergic excitatory pathways in the enteric nervous system in addition to the known vagal pathways.

关 键 词：GHRELIN GHRP-6 胃动素 胃排空 小肠推进 

分 类 号：Q95-3[生物学—动物学] R57[医药卫生—消化系统]