NOK在骨肉瘤细胞系MG63 EMT过程中的作用  

The Roles of NOK in the EMT Process of Osteosarcoma

作　　者：王兆宁 郑立峰 陈岩 王宝 王可 王居正[1] WANG Zhao-ning, ZHENG Li-feng, CHEN Yah, WANG Bao, WANG Ke, WANG Ju-zheng(Department of Orthopnedics, The People＇s liberation nrmy 222 Hospital Jilin, Jinlin, 132011, Chin)

机构地区：[1]解放军第二二二医院骨科,吉林吉林132011

出　　处：《现代生物医学进展》2018年第3期438-441,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81572252)

摘　　要：目的:通过对比和检测人正常成骨细胞系hFOB1.19以及骨肉瘤细胞系MG63中NOK以及EMT标志性分子E-cadherin、Vimitin的mRNA和蛋白表达量,并观察NOK对骨肉瘤细胞系MG63中EMT标志性分子E-cadherin及Vimitin的mRNA和蛋白表达量的影响,探讨NOK在骨肉瘤细胞系MG63 EMT过程中的作用。方法:qRT-PCR、Western blot法检测人正常成骨细胞系hFOB1.19以及骨肉瘤细胞系MG63中NOK、E-cadherin、Vimitin的mRNA和蛋白表达量;构建慢病毒并干扰骨肉瘤细胞系MG63中NOK表达,qRT-PCR、Western blot法检测干扰NOK前后EMT标志性分子E-cadherin及Vimitin的mRNA和蛋白表达。结果:相比于人正常成骨细胞系,NOK、Vimitin的mRNA和蛋白在骨肉瘤细胞系MG63中高表达,E-cadherin的mRNA和蛋白在骨肉瘤细胞系MG63中低表达。慢病毒干扰骨肉瘤细胞中NOK表达后,E-cadherin的mRNA和蛋白表达升高,Vimitin的mRNA和蛋白表达降低。结论:NOK具有促进骨肉瘤细胞系MG63发生EMT过程。Objective： Through comparing and testing the mRNA and protein expressions of NOK and EMT related molecules E-cadherin and Vimitin in normal ossification cell lines hFOB1.19 and osteosarcoma cell lines MG63, and observing the influence of NOK on the expression of E-cadherin and Vimitin in osteosarcoma, and then the role and mechanism of NOK in the EMT process of osteosarcoma will be explored. Methods： qRT-PCR and Western blot analysis were used to detect the NOK, E-cadherin and Vimitin mRNA and protein expression; and then build slow virus and RNA interference NOK expression in osteosarcoma cell lines MG63, and observe the E-cadherin and Vimitin mRNA and protein expression before or after lentivirus vectors for NOK RNA interfering through qRT-PCR and Western blot. Results： Comparing with normal ossification cell lines, the mRNA and protein of NOK and Vimitin are high expressed and E-cadherin mRNA and protein expression are lower in osteosarcoma cell line MG63. Lentivirus vectors interfering NOK could increase E-cadherin mRNA and protein expression, and reduce Vimitin mRNA and protein expression. Conclusion： NOK could promote the EMT process in osteosarcoma cell line MG63.

关 键 词：NOK 骨肉瘤 EMT 

分 类 号：R-33[医药卫生] R738