加压毛细管电色谱法测定复方芦丁片中芦丁和维生素C的含量  

Determination of Rutin and Vitamin C in Compound Rutin Tablets by pCEC

作　　者：张平 陈睿[1] 包综方 刘国峰 李静 王彦[2] 阎超 ZHANG Ping1,2, CHEN Rui1, BAO Zong-fang1, L1U Guo-feng3, LI Jing2,3, WANG Yan2, YAN Chaou2,3(1 Songjiang Institute for Food and Drug Control, Shanghai, 201600, China; 2 School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China; 3 Unimicro Shanghai Technologies, Shanghai, 201203, Chin)

机构地区：[1]上海市松江食品药品检验所,上海201600 [2]上海交通大学药学院药物分析教研室,上海200240 [3]上海通微分析技术有限公司,上海201203

出　　处：《现代生物医学进展》2018年第3期447-452,共6页Progress in Modern Biomedicine

基　　金：上海市科委科研计划项目(15142200200;16142200300);上海市博士后基金项目(14R21422700);上海市引进技术的吸收与创新专项(XC-ZXSJ-02-2016-11)

摘　　要：目的:采用加压毛细管电色谱法测定复方芦丁片中芦丁与维生素C的含量。方法:采用Trisep^(TM)-2100加压毛细管电色谱仪,以C18毛细管色谱柱(300 mm×100μm i.d.,1.8μm)为固定相,流动相为甲醇-乙腈-0.4%磷酸(18:70:12),用三乙胺调节pH值至3.0,总流速为0.05 mL·min^(-1),检测波长为254 nm。结果:芦丁的线性范围为2.0~20μg·mL^(-1)(r^2=0.999);平均回收率为99.4%,RSD为1.2%(n=9);维生素C的线性范围为2.0~50μg·mL^(-1)(r^2=0.999);平均回收率为99.3%,RSD为1.4%(n=9)。另外,本方法与原国标方法进行了比较,结果基本一致。结论:加压毛细管电色谱法可用于复方芦丁片有效成分含量的测定,该方法简单、快速、准确,为复方芦丁片质量控制提供了新检测技术。Objective： A pressurized capillary electrochromatography（p CEC） method has been developed for determination of the active components in compound rutin tablets. Methods： The separation was carried out on a 300 mm×100 μm i.d. capillary packed with1.8 μm ODS by using methanol-acetonitrile-0.4% phosphoric acid（18：70：12） as the mobile phase, and the pH was adjusted to 3.0 with triethylamine. The total flow rate was 0.05 mL·min^-1. The UV absorption detection was carried out at 254 nm. Results： The linear ranges for rutin and vitamin C were 2.0~20 μg·mL^-1（r^2=0.999） and 2.0~50 μg·mL^-1（r^2=0.999）, respectively. Mean recoveries of rutin and vitamin C were 99.4% with relative standard deviation 1.2%（n=9）, and 99.3% with relative standard deviation 1.4%（n=9）, respectively.Besides, the method was compared with the original GB method, the results were basically identical. Conclusions： The method can be used for the determination of compound rutin tablets, which is simple, rapid and accurate. It provides a new detection technology for the quality control of compound rutin tablets.

关 键 词：芦丁 维生素C 加压毛细管电色谱 含量测定 

分 类 号：R-33[医药卫生] R917