玉米中黄曲霉毒素B_1的UHPLC-ESI-MS-MS测定法  

Determination of aflatoxin B_1 in corn by UHPLC-ESI-MS-MS

作　　者：江恩源[1] 劳哲[1] JIANG En-yuan, LAO Zhe(Physical ＆ Chemical Inspection Section of Wuzhou Municipal Center for Disease Control and Prevention, Wuzhou Guangxi, 543002, Chin)

机构地区：[1]梧州市疾病预防控制中心理化检验科,广西梧州543002

出　　处：《职业与健康》2018年第2期178-181,共4页Occupation and Health

摘　　要：目的建立了超高压液相色谱-电喷雾离子源-三重四极杆质谱(UHPLC-ESI-MS-MS)法测定玉米中黄曲霉毒素B1的方法。方法样品用甲醇-水(V∶V=55∶45)提取,离心后经液-液萃取净化,用Shim-pack GISS C18色谱柱(50 mm×2.1 mm,1.9μm)进行色谱分离,以电喷雾正离子多反应监测(MRM)模式进行测定,外标法定量。结果黄曲霉毒素(AFT)B1的最低检出限为0.02μg/kg,平均加标回收率在81.4%~83.4%之间,相对标准偏差(RSD)在3.4%~8.4%之间。结论该方法操作简便、快捷,准确度和灵敏度较高,精密度较好,适用于玉米中黄曲霉毒素B1的测定。[Objective] To establish a method for determination of aflatoxin B1 in corn by ultra-high-pressure liquid chromatographyelectrospray ionization-tandem triple quadrupole mass spectrometry（UHPLC-ESI-MS-MS）.[Methods] The sample was extracted by methanol-water by methanol-water（V∶V=55∶45）,and purified through liquid-liquid extraction after centrifugation.The extract components were separated on the Shim-pack GISS C18 column（50 mm×2.1 mm,1.9 μm） by gradient elution,and detected under electrospray positive ion multiple reaction monitoring（MRM） mode.It was analyzed with external standard method for quantification.[Results] The minimum detection limit for aflatoxin B1 was 0.02 μg/kg.The average recovery rate of standard addition was 81.4%-83.4%.The relative standard deviation（RSD） was 3.4%-8.4%.[Conclusion ] The method is simple,rapid,accurate and sensitive with higher precision.It can be used to determinate the aflatoxin B1 in corn.

关 键 词：超高压液相色谱 三重四极杆质谱 黄曲霉毒素 液-液萃取 

分 类 号：R115[医药卫生—公共卫生与预防医学]