不同免疫表型多发性骨髓瘤患者外周血淋巴细胞亚群分析  

Analysis of peripheral blood lymphocyte subsets in multiple myeloma patients with different immunophenotypes

作　　者：黄国虹[1] 李智伟[1] 王昌敏[1] Huang Guohong, Li Zhiwei, Wang Changmin(Clinical Laboratory Center, People＇s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830011, Chin)

机构地区：[1]新疆维吾尔自治区人民医院临床检验中心,乌鲁木齐830011

出　　处：《肿瘤研究与临床》2018年第3期176-179,共4页Cancer Research and Clinic

摘　　要：目的探讨检测不同免疫表型的多发性骨髓瘤（MM）患者外周血淋巴细胞亚群的临床意义。方法以2016年1月至6月新疆维吾尔自治区人民医院40例MM患者为研究对象，20名健康体检者为对照组，采集骨髓及外周血样本后行流式细胞术进行抗原表达和淋巴细胞亚群检测，分析患者的免疫表型及MM组和对照组之间淋巴细胞亚群各指标的差异。结果CD138、CD38、CD56、CD19、CD20、CD45在MM组和对照组中表达分别为100%（40/40）、100%（40/40）、75%（30/40）、0、20%（8/40）、0和100%（20/20）、100%（20/20）、0、100%（20/20）、100%（20/20）、75%（15/20）；MM组免疫表型可分为CD138＋ CD38＋ CD56- CD19-（10例）和CD138＋ CD38＋ CD56＋ CD19-（30例）两类。MM组中CD3＋[（924±341）个/ml比（1 474±270）个/ml]、CD4＋[（410±163）个/ml比（871±214）个/ml]、CD4＋/CD8＋[（1.0±0.3）个/ml比（1.6±0.5）个/ml]、CD19＋[（72±26）个/ml比（277±89）个/ml]的绝对计数值小于对照组，差异均有统计学意义（均P〈0.05）。MM组中不同免疫表型患者的外周血淋巴细胞亚群各指标绝对计数值间比较，差异均无统计学意义（均P〉0.05）。结论流式细胞术检测可对MM患者进行准确的免疫分型，能更好地区分良、恶性浆细胞，辅助临床对浆细胞骨髓瘤患者进行诊断。结合淋巴细胞亚群分析可反映MM患者的免疫功能状态。ObjectiveTo explore the clinical significance of peripheral blood lymphocytesubsets detection in multiple myeloma （MM） patients with different immunophenotypes.MethodsA total of 40 MM patients in People＇s Hospital of Xinjiang Uygur Autonomous Region from January 2016 to June 2016 were selected as the research objects, and 20 healthy examinations were treated as the control group. Marrow and blood samples were collected to detect the expressions of antigens and peripheral blood lymphocytesubsets by using flow cytometry. Besides, immunophenotype and the differences of lymphocytesubsets between the two groups were analyzed.ResultsThe expression rates of CD138, CD38, CD56, CD19, CD20, CD45 were 100% （40/40）, 100% （40/40）, 75% （30/40）, 0, 20% （8/40）, 0 in MM group and 100% （20/20）, 100% （20/20）, 0, 100% （20/20）, 100% （20/20）, 75% （15/20） in the control group. There were two kinds of immunophenotypes in MM group including CD138＋ CD38＋ CD56- CD19- （10 cases） and CD138＋ CD38＋ CD56＋ CD19- （30 cases）. The absolute value of CD3＋ [（924±341）/ml vs. （1 474±270）/ml], CD4＋ [（410±163）/ml vs. （871±214）/ml], CD4＋/CD8＋ [（1.0±0.3）/ml vs. （1.6±0.5）/ml], CD19＋ [（72±26）/ml vs. （277±89）/ml] was lower in MM group compared with the control group（P 〈 0.05）. There was no statistical difference in the absolute values of peripheral blood lymphocytesubsets for the patients with different immunophenotypes in MM patients （all P 〉 0.05）.ConclusionsFlow cytometry could help to type accurately the different immunophenotypes, distinguish the benign and malignant of plasmocytes and assist the diagnosis for the MM patients. Peripheral blood lymphocytesubsets analysis can be used to reflect the immunity of MM patients.

关 键 词：多发性骨髓瘤 免疫表型 淋巴细胞亚群 流式细胞术 

分 类 号：R733.3[医药卫生—肿瘤]