Rac1基因在子宫内膜癌组织表达及其临床意义  

EXPRESSION OF RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1 IN ENDOMETRIAL CARCINOMA TISSUE AND ITS CLINICAL SIGNIFICANCE

作　　者：宋俊霞[1] 孔令普[1] 杜增利 SONG Junxia, KONG Lingpu, DU Zengli(Department of Obstetrics and Gynecology, Jiaozuo Coal Industry Group Central Hospital, ]iaozuo 454000, Chin)

机构地区：[1]焦作煤业集团中央医院妇产科,河南焦作4540001 [2]焦作煤业集团中央医院麻醉科,河南焦作4540001

出　　处：《精准医学杂志》2017年第6期636-638,641,共4页Journal of Precision Medicine

基　　金：吴阶平医学基金项目(320.6750.14109)

摘　　要：目的探讨Ras相关的C3肉毒素底物1(Rac1)基因在子宫内膜癌组织中表达情况及其临床意义。方法应用实时荧光定量PCR检测96例子宫内膜癌和40例正常子宫内膜组织Rac1基因的表达,并分析其与子宫内膜癌病人生存的关系以及子宫内膜癌病人预后的影响因素。结果子宫内膜癌组织Rac1 mRNA相对表达量为1.75±0.16,显著高于正常子宫内膜组织(1.38±0.14),差异有统计学意义(t=14.095,P<0.01)。子宫内膜癌组织Rac1 mRNA相对表达量与肿瘤分化程度、浸润深度、淋巴结转移和血管浸润有关(t=5.322~9.739,P<0.05)。Kaplan-Meier生存分析显示,Rac1低表达组病人平均生存时间为58.82个月,高表达组为45.98个月,两组比较差异有显著性(χ2=4.972,P<0.05)。Cox比例风险回归模型分析显示,肿瘤分化程度、淋巴结转移和Rac1 mRNA相对表达量是影响子宫内膜癌病人预后的风险因素(HR=2.449~13.582,P<0.05)。结论Rac1基因在子宫内膜癌组织中呈高表达,是影响预后的风险因素之一。Objective To investigate the expression of Ras-related C3 botulinum toxin substrate 1 （Racl） gene in endo metrial carcinoma tissue and its clinical significance. Methods Quantitative real-time PCR was used to measure the expression of the Rac i gene in 96 cases of endometrial carcinoma and 40 cases of normal endometrium tissues. The relationship between the ex- pression of the Racl gene and the survival of patients with endometrial carcinoma, as well as the prognostic factors, was then ana- lyzed. Results The relative expression level of Racl mRNA in endometrial carcinoma tissues was 1.75±0.16, which was signi- ficantly higher than that in the normal endometrial tissues （ 1.38± 0.14） （t= 14. 095, P 〈 0.01 ）. The relative expression level of Racl mRNA in endometrial carcinoma tissues was related to the degree of tumor differentiation, depth of invasion, lymph node metastasis, and blood vessel invasion （t = 5.322 -- 9.739, P〈0.05 ）. Kaplan-Meier survival analysis showed that t he low Rac 1 ex- pression group had a significantly longer mean survival time than the high Rac 1 expression group （58.82 vs 45.98 months, Z2 4.972 ,P〈0.05）. Cox proportional-hazards regression model analysis showed that tumor differentiation degree, lymph node metas- tasis, and the relative expression level of Racl mRNA were prognostic factors in patients with endometrial carcinoma （hazard ratio （HR）=2.449--13.582,P〈O.05）. Conclusion High expression of Racl in endometrial carcinoma tissue is one of the risk fac tors for prognosis.

关 键 词：子宫内膜肿瘤 Ras相关的C3肉毒素底物1 实时聚合酶链反应 预后 

分 类 号：R737.33[医药卫生—肿瘤]