猪圆环病毒2型标签重组病毒构建和免疫试验  

Construction of Tagged Recombinant Porcine Circovirus Type 2 and Their Immunogenicity in Mice

作　　者：华涛[1,2,3] 唐波 常晨[1,2,3] 刘国阳 张雪花[1,2,3] 于漾 侯继波[1,2,3] 张道华 HUA Tao1,2,3, TANG Bo1,2,3, CHANG Chen1,2,3, LIU Guoyang1,2,3,ZHANG Xuehua1,2,3, YU Yang1,2,3 , HOU jibo1,2,3, ZHANG Daohua1,2,3(1.Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Veterinary Immunology ＆ Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014; 2.National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014; 3.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Yangzhou 225009, Chin)

机构地区：[1]江苏省农业科学院动物免疫工程研究所,省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,南京210014 [2]江苏省农业科学院国家兽用生物制品工程技术研究中心,南京210014 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《中国农业科技导报》2018年第4期36-43,共8页Journal of Agricultural Science and Technology

基　　金：江苏省农业自主创新项目[CX(14)5044]; 农业部公益性行业(农业)科研专项经费项目(201303046)资助

摘　　要：猪圆环病毒2型(PCV2)能引起断奶仔猪衰竭综合症,为了获得利于纯化的病毒和新型标记疫苗,采用病毒重组技术,选择利于纯化的6个氨基酸His标签作为标记表位,插入PCV2 Cap蛋白C端,拯救获得重组标记病毒rPCV2-His。间接免疫荧光和Western blot鉴定结果证明重组病毒所含的标签能稳定表达。通过镍柱纯化重组病毒,该病毒获得了纯化。纯化的重组病毒灭活后免疫小鼠,PCV2特异抗体显著提升,且能产生标记免疫抗体,小鼠攻毒显著降低体内PCV2含量。该重组病毒可作为PCV2新型疫苗研究重要材料。Porcine circovirus type 2（ PCV2） can cause postweaning multisystemic wasting syndrome. In order to obtain the purified virus and develop novel marker vaccine against PCV2,a 6-amino-acid His tag was inserted into the C-terminus of capsid protein of PCV2. The recombinant mark virus rPCV2-His expressing the His tag was constructed and rescued. The recombinant marker virus was detected by indirect immunouorescence assay and Western blot,and was purified by nickel column. PCV2 specific antibody was significantly improved and His tag immune antibody was produced in mice inmunized with purified recombinant virus. PCV2 content was significantly decreased in mice inmunized with purified recombinant virus. The findings of this study may be used for the development of a novel PCV2 vaccine.

关 键 词：PCV2 重组病毒 纯化 标记 免疫 

分 类 号：S855.3[农业科学—临床兽医学]