智能化曝气控制A/O工艺活性污泥特性演化对内源反硝化脱氮的作用机制  

Mechanism of Action of Activated Sludge Properties in Nitrogen Removal by Endogenous Denitrification Through an Intelligent Aeration-controlled A/O Process

作　　者：徐旻旸 胡湛波 张穗生[3] 李昊航 XU Min-yang1 , HU Zhan-bo1,2 , ZHANG Sui-sheng3 , LI Hao-hang2(1. School of Resources, Environment and Materials, Guangxi University, Nanning 530004, China; 2. Guangxi Yijiang Environmental Protection Technology Co. , Ltd. , Nanning 530004, China; 3. Department of Agriculture and Environmental Engineering, Guangxi Vocational ＆ Technical College, Nanning 530226, Chin)

机构地区：[1]广西大学资源环境与材料学院,南宁530004 [2]广西益江环保科技股份有限公司,南宁530004 [3]广西职业技术学院农业与环境工程技术系,南宁530226

出　　处：《环境科学》2018年第4期1720-1730,共11页Environmental Science

基　　金：2015年广西科学研究与技术开发计划项目(桂科能1598026-1;桂科攻1598016-3)

摘　　要：将新型智能化曝气控制系统(automatic oxygen supply device,AOSD)应用于A/O工艺中,对比分析AOSD系统曝气模式控制下的A/O工艺(I-A/O)与常规曝气模式A/O工艺(C-A/O)对活性污泥驯化作用、微生物菌群的分布影响,深入探讨IA/O活性污泥特性演化对内源反硝化脱氮的作用机制.结果表明I-A/O系统出水NH_4^+-N、NO_2^--N出现一定积累效应,活性污泥在驯化过程中表现为更为显著的微膨胀,在外源碳素充盈状况下能更充分地将污泥混合液中的溶解性COD(SCOD)富集,将其转化为糖原(Gly),并在外源碳素供应匮乏的状况下激发内源反硝化脱氮反应,内源反硝化速率(EDNR)平均可达0.83 mg·(L·h)^(-1),超过C-A/O水平.通过Illumina HiSeq高通量测序技术对两系统污泥微生物群落结构解析:两系统污泥微生物群落多样性评估区别不明显,而I-A/O污泥中Candidate division TM7大量增殖是构成其丝状菌污泥微膨胀、Gly贮存能力提高的优势功能菌.AOSD的供氧模式使活性污泥特性与微生物群落在适应新环境上发生了特异性变化,好氧异养菌代谢活性下降,内源反硝化脱氮途径的强化使I-A/O实现了一种总体低氧需求的动态平衡状态.In order to evaluate the mechanism of action of activated sludge properties in nitrogen removal by endogenous denitrification（ henceforth EDNR）,a new kind of automatic oxygen supply device（ AOSD）,was applied to the A/O process. The domestication effect of the aeration mode on the activated sludge properties and microbial communities was investigated under the intelligent aerationcontrolled A/O process（ I-A/O） and the continuous aeration A/O process（ C-A/O）. The results demonstrated that the effluent NH_4~＋-N and NO_2^--N components showed obvious accumulation efficiencies and activated sludge generated conspicuous limited bulking in the I-A/O process. Domesticated sludge in the I-A/O process was able to enrich more SCOD to transfer into the polymeric substances as Gly,under a rich exogenous carbon supply state,and stimulated nitrogen removal by endogenous denitrifying under a scarce exogenous carbon supply state. The EDNR rate went up to 0. 83 mg·（ L·h）^-1 in the I-A/O process,which was more than that achieved by the CA/O process. The microbial communities in the two processes were evaluated by the Illumina Hi Seq high-throughput sequencing technology. The results showed that there was no obvious difference in the sludge microbial community diversity between the two processes,but the Candidate division TM7 proliferated in the I-A/O process,and become the abundant taxa to prompt limited filamentous sludge bulking and Gly storage capability enhancement. The oxygen supply mode of AOSD made the activated sludge properties and microbial communities to be screened selectively in the new environment,aerobic heterotrophic bacterial activity to decline,and endogenous denitrifying action to strengthen,which made the I-A/O process implement a kind of dynamic balanced state that limited the DO demand.

关 键 词：智能化曝气控制系统 A/O工艺 活性污泥特性 碳源转化 内源反硝化脱氮 ILLUMINA HiSeq高通量测序 

分 类 号：X703.1[环境科学与工程—环境工程]