复合菌株YH01+YH02强化SBR好氧反硝化脱氮及菌群结构分析  

Aerobic Denitrification and Microbial Community Shift in SBR Bioaugmented with Strains YH01 and YH02

作　　者：陈海升 曹刚 张迪 黄郑郑 莫测辉 CHEN Hai-sheng1, CAO Gang1,2 , ZHANG Di1 , HUANG Zheng-zheng1 , MO Ce-hui1,2(1. School of Environment, Jinan University, Guangzhou 510630, China; 2. Guangdong Engineering Center for Environment Contamination Control and Restorative Materials, Guangzhou 510630, Chin)

机构地区：[1]暨南大学环境学院,广州510630 [2]广东省污染控制与修复材料工程技术中心,广州510630

出　　处：《环境科学》2018年第4期1773-1781,共9页Environmental Science

基　　金：国家自然科学基金委员会-广东省政府联合基金重点项目(U1501233);广东省自然科学基金研究团队项目(2016A030312009);广东省环境污染控制与修复材料工程技术研究中心建设项目(2015B090903070)

摘　　要：为探讨复合菌株强化好氧反硝化的脱氮特性以及揭示菌群结构动态演替与生物反应器运行效率之间的联系,以硝态氮为底物,研究了异养硝化-好氧反硝化复合菌株Delftia sp.YH01+Acidovorax sp.YH02强化好氧SBR的脱氮特性,并借助高通量测序技术对强化过程中的菌群结构进行了分析.结果表明,经复合菌株强化后,反应器对NO_3^--N、TN和COD去除率分别提高了12.1%、9.2%和9.4%;复合菌株YH01+YH02的加入对菌群结构产生了较大的影响,菌群结构在属水平上丰度呈上升趋势,多样性呈下降趋势;PCA主成分析和UPGMA聚类分析大致把反应器运行过程分成4个阶段;Delftia和Acidovorax菌的丰度随着反应器呈上升趋势,复合菌株YH01+YH02在SBR反应器内具有良好的适应能力,对好氧反硝化起着非常重要的作用.The enhanced aerobic denitrification capability of the mixed strains YH01 ＋ YH02 in utilizing potassium nitrate,and the dynamic changes in the microbial community component during the period of operation,were evaluated. The microbial community in different stages of the SBR was analyzed by using high-throughput sequencing technology after inoculation with YH01 ＋ YH02. The results showed that the NO3^--N,TN,and COD removal efficiencies increased by 12. 1%,9. 2%,and 9. 4%,respectively. The relative abundances of the microbes in the microbial community increased at the genus level,and the diversity in the microbial community decreased after enhancement. Principal component analysis and UPGMA analysis revealed that the period of SBR operation was roughly divined into four phases. The relative abundances of Delftia and Acidovorax increased during the period of operation,and YH01 ＋ YH02 exhibited excellent compatibility with the SBR ecosystem and played an important part in aerobic denitrification.

关 键 词：异养硝化-好氧反硝化 复合菌株 SBR反应器 高通量测序技术 菌群结构 

分 类 号：X172[环境科学与工程—环境科学]