氮磷失衡下膨胀污泥性能及膨胀菌群落结构变化  

Performance,Sludge Characteristics,and the Microbial Community Dynamics of Bulking Sludge Under Different Nitrogen and Phosphorus Imbalances

作　　者：贺雪濛 丁丽丽[1] 张璐璐 顾卓江 任洪强[1] HE Xue-meng1, DING Li-li1 ,ZHANG Lu-lu1 , GU Zhuo-jiang2 , REN Hong-qiang 1.(1. State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, China; 2. Hisun Pharmaceutical Company Limited, Hangzhou 311404, Chin)

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,南京210023 [2]浙江海正药业有限公司,杭州311404

出　　处：《环境科学》2018年第4期1782-1793,共12页Environmental Science

基　　金：国家科技支撑计划项目(2014BAC08B04)

摘　　要：采用高通量测序技术(16S rRNA)、高效液相色谱(HPLC)等方法,重点比较了不同氮磷失衡条件下膨胀污泥性能及群落结构(含膨胀菌)的变化.结果表明,以膨胀污泥为接种污泥(A/O工艺),经过一段时间培养单独限氮(RN)组的沉降性能可恢复到SVI<150 mL·g^(-1),单独限磷(RP)组SVI指数呈现相对较弱的改善趋势;正常C/N/P(100∶5∶1)条件的R0会导致污泥SVI指数最高(SVI=1 496 mL·g^(-1)),其次为同时限制氮磷组(RNP).正常营养条件下膨胀污泥脂多糖相对含量(LPS/MLVSS)与沉降性能SVI的皮尔逊相关性分析结果存在显著负相关(r=-0.625,P<0.05);营养限制条件下污泥(膨胀期)LPS对生物量的指示性更为准确.Thiothrix为4组反应器中主导膨胀菌,PCo A的结果显示Ⅱ、Ⅲ阶段中存在氮限制的组(RNP、RN)群落迁移变化较大,RDA结果显示Thiothrix与污泥沉降性能及比耗氧速率呈现显著相关.In this paper,the performance,characteristics of the bulking sludge,and the variations in the microbial community（ including the bulking bacteria） under different nitrogen and phosphorus imbalances were compared,using high-throughput sequencing（ 16 S rRNA） and the high performance liquid chromatography（ HPLC） technology. The results showed that after seeding bulking sludge in the A/O process and operating for a period of time,the sludge settleability of the nitrogen limitation alone reactor（ RN） could recover to normal [sludge volume index（ SVI） 150 mL·g^-1],while the SVI of the phosphorus limitation alone reactor（ RP）improved slightly; the control reactor（ R0,C/N/P = 100/5/1） exhibited the highest SVI index（ SVI = 1 496 mL·g^-1）,followed by the reactor of simultaneous nitrogen and phosphorus limitations（ R（NP））. Under normal nutritional conditions,Pearson correlation analysis showed a significant negative correlation between the lipopolysaccharide（ LPS） relative content（ LPS/MLVSS） and the settleability of bulking sludge（ r =-0. 625,P〈0. 05）,while under nutrient limitation conditions,LPS showed high accuracy in reflecting the biomass of the activated sludge. Thiothrix was the dominant bulking bacteria in all the reactors. PCo A analysis showed that the migration of the community in the reactors experienced nitrogen limitation（ RNP,RN） changes greatly during the stages Ⅱ andⅢ,while RDA analysis showed that the correlation of Thiothrix with the settling performance and oxygen consumption rate was significant.

关 键 词：膨胀污泥 氮磷失衡 沉降性能 脂多糖(LPS) 群落结构 

分 类 号：X172[环境科学与工程—环境科学] X703