茚三酮比色法和旋光度法测定谷氨酸制备焦谷氨酸的转化率(英文)  

Determination of Glutamic Acid Conversion Rate in the Preparation Process of Pyroglutamic Acid by Ninhydrin Colorimetryand Polarimetry

作　　者：董恺洁 于佳 郭群群 胡迎芬 赵爱云 魏玉西 DONGKai-jie,YU J ia,GUOQun-qun,HU Ying-fen,ZHAO Ai-yun,WEI Yu-xi(College of Life Sciences, Qingdao Universi ty, Qingdao 266071, Chin)

机构地区：[1]青岛大学生命科学学院,青岛266071

出　　处：《青岛大学学报（自然科学版）》2018年第1期40-45,共6页Journal of Qingdao University(Natural Science Edition)

基　　金：山东省重点研发项目(批准号:2016ZDJS06A01)资助

摘　　要：研究了通过茚三酮比色法和旋光度法测定谷氨酸制备焦谷氨酸的转化率。通过紫外可见分光光度计在568nm,pH6.0状态下检测与茚三酮反应的谷氨酸和焦谷氨酸的含量。研究结果表明,谷氨酸具有较大的吸收峰,但焦谷氨酸吸光度为零。根据谷氨酸的标准曲线方程,可以计算其后反应溶液中的含量进而计算出转化率。基于谷氨酸和焦谷氨酸的旋光度—转化率工作曲线,可直接计算谷氨酸制备焦谷氨酸的转化率。结果表明,与高效液相色谱法相比,茚三酮比色法和旋光度法具有操作简单、快速、灵敏度高和成本低的优点。Ninhydrin colorimetry and polarimetry were studied to determine the conversion of PCA prepared by glutamic acid.The content of Glu and PCA reacted with ninhydrin was detected by ultraviolet-visible spectrophotometer（UV/VIS）at 568 nm,pH6.0.It was showed that Glu has a larger absorption peak,however the absorbance of PCA were zero.According to the standard curve equation of Glu,its content in the after-reacting solution can be calculated.Besides,the optimal rotation-conversion rate curves,by which the remaining Glu of the after-reacting can be calculated,were established on the basis of Glu and PCA optical rotation.The results indicated that compared with HPLC,ninhydrin colorimetry and polarimetry were simple,fast,highly sensitive and low-cost.

关 键 词：谷氨酸 焦谷氨酸 茚三酮比色法 旋光度法 

分 类 号：Q819[生物学—生物工程]