菊芋多糖对2型糖尿病大鼠胰岛细胞形态与功能影响  

Influences of Jerusalem artichoke polysaccharide on morphology and function of islet cells of type Ⅱ diabetes rats

作　　者：黄晓东 王艳春 李晓光 任旷 沈楠[3] HUANG Xiao-dong, WANG Yan-chun, LI Xiao-guang, et al(College of Basic Medicine, Jilin Medical University, Jilin, Jilin Province 132013, Chin)

机构地区：[1]吉林医药学院基础医学院,吉林吉林132013 [2]吉林医药学院药学院 [3]吉林医药学院实验中心

出　　处：《中国公共卫生》2018年第3期365-368,共4页Chinese Journal of Public Health

基　　金：吉林市科技计划项目(2015233012)

摘　　要：目的探讨菊芋多糖对2型糖尿病大鼠胰岛细胞形态与功能影响及机制。方法使用高脂饮食并结合小剂量链脲佐菌素制备2型糖尿病大鼠模型,将造模成功大鼠随机分为对照组、模型组、二甲双胍组(阳性对照)、菊芋多糖低、中、高剂量组(20、40、80 mg/kg),连续灌胃8周后检测大鼠空腹血糖、血清胰岛素水平;苏木精–伊红染色观察胰腺组织病理损伤程度;免疫组化法检测大鼠胰腺细胞胰岛素的表达。结果与对照组比较,模型组大鼠空腹血糖[(18.24±1.32)mmol/L]明显升高,血清胰岛素水平[(14.89±1.76)ng/m L]明显下降,差异均有统计学意义(均P<0.05);与模型组比较,40、80 mg/kg菊芋多糖组大鼠空腹血糖[分别为(10.77±0.12)、(8.56±1.45)mmol/L]明显下降,80 mg/kg菊芋多糖组大鼠血清胰岛素水平[(16.46±1.88)ng/m L]明显升高,差异均有统计学意义(均P<0.05);菊芋多糖可减轻糖尿病大鼠胰腺组织病理学损伤程度,还可提高糖尿病大鼠胰腺组织胰岛素表达。结论菊芋多糖可减轻2型糖尿病大鼠胰岛素抵抗,减轻胰岛β细胞损伤,增加胰岛素表达,从而发挥降血糖作用。Objective To explore effects of Jerusalem artichoke polysaccharide （JAP） on morphology and function of islet cells of type II diabetes rats and the mechanism of the effects. Methods Type 2 diabetes mellitus （T2DM） rat model was established by feeding the Wistar rats with high-fat feeds combined with the administration of small dose of streptozotocin （STZ）; the rats with routine feeds were used as a normal control group. Then the T2DM model rats were randomly assigned into a positive control group administered with dimethyldiguanide, three JAP groups administered with JAP at the dosages of 20, 40, and 80 mg·kg-1, and a model group treated with double distilled water; all the administrations were performed via gavage once a day continuously for 8 weeks. Fasting plasma glucose （FPG） and fasting insulin level （FINS） of all the rats were measured at the end of the treatments. The morphologic changes of pancreatic tissue was observed with hematoxylin-eosin staining and the expression of insulin of pancreas ceils of the rats was detected with immunohistochemical method. Results Compared with those of the normal control rats, the FPG of model rats （18.24 ± 1.32 mmol/L） was significantly increased but the FINS （14.89 ±1.76 ng/mL） was decreased significantly （both P 〈 0.05）. Compared with those of the model rats, the FPG was significantly decreased to 10.77 ± 0.12 mmol/L and 8.56 ± 1.45 mmol/L for the rats treated with 40 and 80 mg·kg-1 JAP （both P 〈 0.05）; while the FINS was significantly increased to 16.46 ± 1.88 ng·ml-1 for the rats treated with 80 mg·kg-1 JAP （P 〈 0.05）. Alleviated pathological changes and increased insulin expression were observed in the pancreatic tissues of the rats treated with JAP. Conclusion JAP has hypoglycemic effect through reducing insulin resistance, alleviating damage of islet β cells, and increasing the expression of insulin in rats with type type 2 diabetes mellitus.

关 键 词：菊芋多糖 2型糖尿病 降血糖 胰岛素 胰岛Β细胞 

分 类 号：R332[医药卫生—人体生理学]