B族维生素片中维生素B_(12)电感耦合等离子体质谱法间接测定  

Indirect detection of VB_(12) in vitamin B complex tablets with inductively coupled plasma mass spectrometry

作　　者：王国玲[1] 邵立君[1] 聂宏骞[1] 杨路平[1] 王锡宁[1] WANG Guo-ling, SHAO Li-jun, NIE Hong-qian, et al(Shandong Provincial Center for Disease Control and Prevention, Ji＇nan, Shandong Province 250014, Chin)

机构地区：[1]山东省疾病预防控制中心,济南250014

出　　处：《中国公共卫生》2018年第3期462-464,共3页Chinese Journal of Public Health

摘　　要：目的建立电感耦合等离子体质谱(ICP-MS)法间接测定B族维生素片中维生素B_(12)含量的方法。方法样品经微波消解后,采用在线加入内标校正基体效应,用ICP-MS测定钴离子浓度间接测定样品中维生素B_(12)的含量。结果本方法在1.0~100.0μg/L线性范围内具有良好的线性关系(r>0.999),加标回收率为95.0%~104.0%,相对标准偏差(RSD)为1.17%~3.03%,方法检出限为0.014 mg/kg,定量下限为0.041 mg/kg。结论本方法简单、灵敏、准确,适用于B族维生素片中维生素B_(12)的测定。Objective To establish an inductively coupled plasma mass spectrometry （ICP-MS） method for indirect detection of vitamin B12 content in vitamin B complex tablets.Methods The sample was pretreated with microwave digestion and the matrix effect was corrected with the in-line addition of internal standards. Vitamin B12 was determined indirectly with the detection of cobalt ions in the sample.Results The linearity range of the established method was satisfying for the concentration of 1.0—100.0 g/L, with the the correlation coefficients of greater than 0.999. The average recoveries were 95.0%—104.0%, with a relative standard deviation of 1.17%—3.03%. The limit of detection （LOD） was 0.014 mg/kg and the limit of quantification （LOQ） was 0.041 mg/kg.Conclusion The method is simple, sensitive, accurate, and applicable for the detection of vitamin B12 content in vitamin B complex tablets.

关 键 词：电感耦合等离子体质谱(ICP—MS) 间接测定 B族维生素片 维生素B12 

分 类 号：R155.5[医药卫生—营养与食品卫生学]