漆酶催化4-氨基苯酚聚合  

Laccase-catalyzed Polymerization of 4-Aminophenol

作　　者：朱洪玲 傅佳佳[2,3] 许波 王强 Artur Cavaco Paulo[1,3] 苏静 ZHU Hong-ling1,3, FU Jia-jia2,3, XU Bo1,3, WANG Qiang1,3, Artur Cavaco Paulo1,3, SU Jing1,3(1. International Joint Research Laboratory for Textile and Fiber Bioprocesses, Textile ＆ Garment Institute, Jiangnan University, Wuxi 214122, Jiangsu, China; 2. Jiangsu Engineering Technology Research Center for Funetional Textiles Textile ＆ Garment Institute ,Jiangnan University, Wuxi 214122 ,Jiangsu , China ;3. Key Laboratory of Eco- Textiles Ministry of Education Textile ＆ Garment Institute, Jiangnan University, Wuxi 214122, Jiangsu, China)

机构地区：[1]江南大学纺织服装学院纺织生物技术国际联合实验室,江苏无锡214122 [2]江南大学纺织服装学院江苏省功能纺织品功能技术研究中心,江苏无锡214122 [3]江南大学纺织服装学院生态纺织教育部重点实验室,江苏无锡214122

出　　处：《精细化工》2018年第4期620-624,共5页Fine Chemicals

基　　金：国家自然科学基金(31470509,31201134);江苏省产学研前瞻性研究项目(BY2016022-23);中央高校基本科研业务费专项资金资助(JUSRP51622A);2016年度江苏省普通高校学术研究生科研创新计划项目(KYLX16_0788)~~

摘　　要：在微型反应器（40℃）中,将漆酶加入醋酸缓冲溶液（pH=5.0）中催化4-氨基苯酚聚合,持续反应10 h后得到黑色粉末状聚合物。通过UV-Vis观察了4-氨基苯酚及其聚合物吸收光谱的变化,采用FTIR、1HNMR、MALDI-TOF MS、TGA和DSC对产物的结构和性能进行了表征。结果表明：所得聚合物在氯仿中不溶,在丙酮、四氢呋喃、N,N-二甲基甲酰胺和二甲基亚砜中均有良好的溶解性。UV-Vis、FTIR和1HNMR结果显示：聚合物中酚羟基保留,氨基参与反应,苯环上的氢原子部分参与反应,并且推测了聚合物的结构。MALDI-TOF MS、TGA和DSC结果显示：聚合物为低聚物,相对分子质量分布在300-1 300 Da,聚合度为3-12。聚4-氨基苯酚的分解温度为223℃,具有良好的耐热性能。Laccase-catalyzed polymerization of 4-aminophenol was conducted in acetate buffer solution（pH = 5. 0） at 40 ℃. After continuous reaction for 10 h,black solid powers were obtained. UV-Vis spectra were used to observe the absorption spectra changes of 4-aminophenol and its polymer. The structure and properties of product were characterized by means of FTIR,1 HNMR,MALDI-TOF MS,TGA and DSC. The results showed that the as-prepared polymer was insoluble in chloroform,and soluble in acetone,tetrahydrofuran,N,N-dimethylformamide and dimethyl sulfoxide. UV-Vis,FTIR and1 HNMR spectra of product revealed that the synthetic polymer contained phenolic hydroxyl groups,the amine group of 4-aminophenol participated in the polymerization reaction,and partial hydrogen atoms on the benzene ring involved in the reaction. The possible structures of polymer were speculated. In addition,the obtained poly（4-aminophenol） was an oligomer with a molecular weight ranging from 300 to 1 300 Da,its degree of polymerization was 3 to 12. The polymer had excellent heat resistance with a decomposition temperature of 223 ℃.

关 键 词：漆酶 4-氨基苯酚 聚合 质谱 催化技术 

分 类 号：TQ246.35[化学工程—有机化工]