海桐种子醇提物中挥发性成分的GC-MS分析及抗内毒素活性测定  

GC-MS Analysis of Volatile Components in Ethanol Extract from Seeds of Pittosporum Tobira Ait. and Determination of Anti-endotoxin Activity

作　　者：宋晓凯[1] 张秀莲 SONG Xiaokai, ZHANG Xiulian(Department of Pharmaceutic Preparation, College of Pharmacy, Huaihai Institute of Technology, Lianyungang 222005, Chin)

机构地区：[1]淮海工学院药物制剂系,江苏连云港222005

出　　处：《中国现代应用药学》2018年第2期248-251,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：江苏省海洋资源开发研究院开放课题(JSIMR201504)

摘　　要：目的研究海桐种子乙醇总提取物(ethanol extract from seeds of Pittosporum tobira Ait,EESPTA)的挥发性成分及相对含量,考察其抗内毒素活性。方法以水蒸气蒸馏法提取EESPTA的挥发性成分,用气-质联用技术初步分析鉴定,并用峰面积归一化法测定各成分相对含量,采用终点基质显色法鲎实验检测其对细菌内毒素的清除效果,考察其抗内毒素活性。结果共检测出并初步分析鉴定32个色谱峰,占该挥发性成分总量的99.82%。在EESPTA的挥发性成分中,1R,4S,7S,11R-2,2,4,8-四甲基三环[5.3.1.0(4,11)]十一碳-8-烯含量高达54.78%,其他含量较高的成分有2-亚甲基-胆甾烷-3-醇(7.27%)、蛇麻烷-1,6-二烯-3-醇(6.23%)、4-甲基-5-癸醇(4.11%)、绿花白千层醇(3.86%)、别香橙烯(3.80%)、反式-2,3-二甲基丙烯酸(3.50%)、二氢-噻喃-3,5-二醇-四氢-4-甲基-4-氮-二乙酸乙酯(2.04%)等成分。结论 EESPTA具有较明显的抗细菌内毒素活性,并呈浓度依赖关系。为进一步开发利用海桐药用价值提供了科学依据。OBJECTIVE To analyze the volatile components in ethanol extract from seeds of Pittosporum tobira Ait.（EESPTA） and investigate the anti-endotoxin activity of EESPTA in vitro.METHODS The volatile components in EESPTA were extracted with steam distillation method and then analysized by GC-MS.The relative content of each component was calculated by peak area normalization.The anti-endotoxin activity of EESPTA in vitro was investigated by CEPTAL（chromogenic end-point tachypleus amebocyte lysate assay）.RESULTS The 32 peaks were detected and then identified preliminary,which made up 99.82% of the total volatile components.The anti-endotoxin activity of EESPTA in vitro was observed in concentration-dependent manner from CEPTAL assay.The highest content constituent was 1R,4S,7S,11R-2,2,4,8-tetramethyltricyclo [5.3.1.0（4,11）] undec-8-ene（54.78%）,and other relative high content constituents were 2-methylene-cholestan-3-ol（7.27%）,humulane-1,6-dien-3-ol（6.23%）,4-methyl-5-decanol（4.11%）,viridiflorol（3.86%）,Alloaromadendrene（3.80%）,2-methyl-2-butenoic acid（3.50%）,et al.CONCLUSION EESPTA has obvious anti-endotoxin activity with a concentration dependence.This study provides a scientific basis for further development and utilization of Pittosporum tobira Ait..

关 键 词：海桐种子 挥发性成分 GC-MS分析 抗内毒素作用 

分 类 号：R971.101[医药卫生—药品]