Semaphorin5A在大肠癌组织中的表达及临床意义  

The expression and clinical significance of Semaphorin5A in colorectal carcinoma

作　　者：史长山 王兴尧 王晓光[1] 吴德辉 解辉[2] SHI Chang-shan1, WANG Xing-yao1, WANG Xiao-guang1, WU De-hui1, XIE Hui2(1. Department of gastroenterolog, Tiefa Coal Industry（Group） General Hospital, Tieling 112700; 2.Department of Histology and Embryology, Shengyang Medical College, Shengyang 110034, China)

机构地区：[1]铁法煤业(集团)总医院消化内科,辽宁铁岭112700 [2]沈阳医学院组织胚胎学教研室,辽宁沈阳110034

出　　处：《解剖科学进展》2018年第2期146-148,共3页Progress of Anatomical Sciences

基　　金：辽宁省科技厅博士启动基金(20131114)

摘　　要：目的检测神经导向分子Semaphorin5A在大肠癌组织中表达,并探讨其在大肠癌发生发展中的意义。方法选取43例大肠癌组织和22例正常大肠粘膜组织,采用免疫组织化学、Western blot和real-time PCR方法检测Semaphorin5A在大肠癌组织及其正常大肠粘膜组织中的表达,并分析其与临床病理特征之间的相关性。结果大肠癌组织中Semaphorin5A的蛋白与mRNA表达水平显著高于正常大肠粘膜组织(P<0.05);Semaphorin5A的表达与大肠癌Dukes分期、淋巴结转移、组织学分型有关,与年龄、性别无关。结论Semaphorin5A可能与大肠癌的发生发展密切相关。Objective To detect the expression of Semaphorin5A in colorectal carcinoma and to explore its significance in the development of colorectal cancer. Methods The expression of Semaphorin5A in 43 cases of coloreetal carcinoma and 22 cases of normal eolorectal mucosa was detected by immunohistochemistry, Western blot and real-time PCR, and the relationship between Semaphorin5A and clinical pathological features of colorectal carcinoma was analyzed. Results The expression level of Semaphorin5A protein and mRNA was significantly higher in colorectal cancer tissues than in normal colorectal mucosa tissues, correlated to Dukes stage, lymph node metastasis and histological type of colorectal cancer, hut not to age and gender. Conclusion Semaphorin5A/night be involved in the pathogenesis of colorectal cancer.

关 键 词：Semaphorin5A 大肠癌 DUKES分期 淋巴结转移 

分 类 号：R735.3[医药卫生—肿瘤]