荧光在循环肿瘤细胞鉴定中的应用  

Application of fluorescence in the identification of circulating tumor cells

作　　者：于华杰 韩露[1] 欧洋[1] 李胜[2] 高德海[2] Yu Huajie , Han Lu, Ou Yang, Li Sheng, Gao Dehai.(School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, University of Ji＇nan, Ji＇nan 250000, Chin)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,250000 [2]山东省医学科学院山东省药物研究院

出　　处：《国际肿瘤学杂志》2018年第1期35-38,共4页Journal of International Oncology

摘　　要：当前鉴定循环肿瘤细胞的方法很多，荧光在循环肿瘤细胞鉴定中有着广泛的应用。用含有荧光基团的抗体、探针和适体等标记循环肿瘤细胞，可以更直观地对被标记细胞进行观察和计数，细胞因子、CD45和荧光原位杂交等被广泛应用于各种循环肿瘤细胞相关试验的鉴定环节中。近年来人们探索了利用荧光探针直接鉴定循环肿瘤细胞的可行性，随着生物荧光探针和共聚焦显微镜光学切片成像技术的发展，未来有可能利用荧光探针直接鉴定循环肿瘤细胞。There are many ways to identify circulating tumor cells in the current. Fluorescence has a wide range of applications in the identification of circulating tumor cells. The labeled cells can be observed and counted more intuitively by labeling the tumor cells with fluorescent group containing antibodies, probes and aptamers, and cytokine, CD45 and fluorescence in situ hybridization are widely used in the identification of various circulating tumor cell related tests. In recent years, people have explored the feasibility of using fluorescent probes to directly identify circulating tumor cells. With the development of biopsy probes and optical sectioning imaging technology of confocal microscopy, it is possible to directly identify circulating tumor cells with fluorescent probes in the future.

关 键 词：肿瘤循环细胞 荧光 原位杂交 荧光 探针 

分 类 号：R73-3[医药卫生—肿瘤]