Role of Glutamine in the Mediation of E-cadherin, p120-catenin and Inflammation in Ventilator-induced Lung Injury  

作　　者：Jian-Lei Qiu Bai-Ling Song Yu-Juan Wang Fu-Tao Zhang Yue-Lan Wang 

机构地区：[1]Department of Anesthesiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, China [2]Department of Anesthesiology, Dezhou People's Hospital, Dezhou, Shandong 253014, China [3]Department of Surgery, Rizhao People's Hospital, Rizhao, Shandong 276826, China [4]Department of Anesthesiology, Rizhao People's Hospital, Rizhao, Shandong 276826, China [5]Department of Emergency, Dezhou People's Hospital, Dezhou, Shandong 253014, China

出　　处：《Chinese Medical Journal》2018年第7期804-812,共9页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （Nos. 81570074, 81770076）.

摘　　要：Background： Ventilator-induced lung injury （VILI） is commonly associated with barrier dysfunction and inflammation reaction. Glutamine could ameliorate VILI, but its role has not been fully elucidated, This study examined the relationship between inflammatory cytokines （interleukin JILl-6, tumor necrosis factor [TNF]-α, and IL-10） and adherens junctions （E-cadherin, p 120-catenin）, which were ameliorated by glutamine in VILI, both in vitro and in vivo. Methods： For the in vivo study, 30 healthy C57BL/6 mice weighing 25-30 g were randomly divided into five groups with random number table （n = 6 in each group）： control （Group C）; low tidal volume （Group L）; low tidal volume ＋ glutamine （Group L ＋ G）; high tidal volume （Group H）; and high tidal volume ＋ glutamine （Group H ＋ G）. Mice in all groups, except Group C, underwent mechanical ventilation for 4 h. For the in vitro study, mouse lung epithelial 12 （MLE- 12） cells pretreated with glutamine underwent cyclic stretching at 20% for 4 h. Cell lysate and lung tissue were obtained to detect the junction proteins, inflammatory cytokines, and lung pathological changes by the Western blotting, cytokine assay, hematoxylin and eosin staining, and immunofluorescence. Results： In vivo, compared with Group C, total cell counts （t= -28.182, P 〈 0.01）, the percentage of neutrophils （t = -28.095, P 〈 0.01）, IL-6 （t = -28.296, P 〈 0.01 ）, and TNF-α（t = - 19.812, P 〈 0.01 ） in bronchoalveolar lavage （BAL） fluid, lung injury scores （t = -6.708, P 〈 0.01）, and the wet-to-dry ratio （t = - 15.595, P 〈 0.01 ） were increased in Group H; IL- 10 in BAL fluid （t = 9.093, P 〈 0.01 ） and the expression of E-cadherin （t= 10.044, P 〈 0.01） and p120-catenin （t = 13.218, P 〈 0.01） were decreased in Group H. Compared with Group H, total cell counts （t - 14.844, P 〈 0.01 ）, the percentage of neutrophils （t = 18.077, P 〈 0.0 l ）, IL-6 （t - 18.007, P 〈 0.01 ）, and TNF-Background： Ventilator-induced lung injury （VILI） is commonly associated with barrier dysfunction and inflammation reaction. Glutamine could ameliorate VILI, but its role has not been fully elucidated, This study examined the relationship between inflammatory cytokines （interleukin JILl-6, tumor necrosis factor [TNF]-α, and IL-10） and adherens junctions （E-cadherin, p 120-catenin）, which were ameliorated by glutamine in VILI, both in vitro and in vivo. Methods： For the in vivo study, 30 healthy C57BL/6 mice weighing 25-30 g were randomly divided into five groups with random number table （n = 6 in each group）： control （Group C）; low tidal volume （Group L）; low tidal volume ＋ glutamine （Group L ＋ G）; high tidal volume （Group H）; and high tidal volume ＋ glutamine （Group H ＋ G）. Mice in all groups, except Group C, underwent mechanical ventilation for 4 h. For the in vitro study, mouse lung epithelial 12 （MLE- 12） cells pretreated with glutamine underwent cyclic stretching at 20% for 4 h. Cell lysate and lung tissue were obtained to detect the junction proteins, inflammatory cytokines, and lung pathological changes by the Western blotting, cytokine assay, hematoxylin and eosin staining, and immunofluorescence. Results： In vivo, compared with Group C, total cell counts （t= -28.182, P 〈 0.01）, the percentage of neutrophils （t = -28.095, P 〈 0.01）, IL-6 （t = -28.296, P 〈 0.01 ）, and TNF-α（t = - 19.812, P 〈 0.01 ） in bronchoalveolar lavage （BAL） fluid, lung injury scores （t = -6.708, P 〈 0.01）, and the wet-to-dry ratio （t = - 15.595, P 〈 0.01 ） were increased in Group H; IL- 10 in BAL fluid （t = 9.093, P 〈 0.01 ） and the expression of E-cadherin （t= 10.044, P 〈 0.01） and p120-catenin （t = 13.218, P 〈 0.01） were decreased in Group H. Compared with Group H, total cell counts （t - 14.844, P 〈 0.01 ）, the percentage of neutrophils （t = 18.077, P 〈 0.0 l ）, IL-6 （t - 18.007, P 〈 0.01 ）, and TNF-

关 键 词：Adherens Junctions GLUTAMINE Inflammatory Cytokines Ventilation-induced Lung Injury 

分 类 号：S858.31[农业科学—临床兽医学] TD724[农业科学—兽医学]