重组人肿瘤坏死因子α对胸苷磷酸化酶的调节及其对卡培他滨抑制宫颈癌细胞SiHa作用的研究  

Experimental studies on the regulatory effect of rhTNF-α on thymidine phosphorylase and effects of rhTNF-α on inhibiting effect on cervical cancer SiHa cells by Capecitabine

作　　者：彭莉 周勇军[1] Yongjun(Department of Gynecology, Yiyang Central Hospital, Yiyang 413000, Chin)

机构地区：[1]湖南省益阳市中心医院妇科,413000

出　　处：《中国实用医刊》2018年第3期98-101,共4页Chinese Journal of Practical Medicine

摘　　要：目的 探讨重组人肿瘤坏死因子-α（rhTNF-α）对胸苷磷酸化酶（TP）的调节及其对卡培他滨抑制宫颈癌细胞SiHa的作用机制.方法 以人宫颈癌细胞SiHa为细胞模型,采用不同浓度的rhTNF-α作用于体外培养的宫颈癌细胞SiHa,以MTT法检测细胞生长抑制率,用ELISA法检测TP水平.结果 rhTNF-α能显著抑制宫颈癌细胞SiHa的生长,呈现明显的量-效关系和时-效关系;随着rhTNF-α浓度的增加,TP表达水平逐步升高,差异有统计学意义（F =26.87,P＜0.01）;rhTNF-α和卡培他滨具有明显的协同作用,并且有剂量依赖性,各药物处理组与对照组比较差异有统计学意义（F=30.56,P＜0.05）.结论 rhTNF-α在体外能有效抑制宫颈癌细胞SiHa增殖,能剂量依赖性地上调TP的表达,增强卡培他滨抗宫颈癌细胞的活性.Objective To investigate the regulatory effect of recombination human tumor necrosis factor-α （rhTNF-α） on thymidine phosphorylase and effect of rhTNF-α on inhibiting effect of capectiabine on cervical cancer SiHa cells.Methods The SiHa human cervical cancer cell line cultured in vitro were given different concentrations of rhTNF-α;and the cell growth inhibiting ratio of SiHa cells were assessed by MTT assay,the level of thymidine phsohphorylase was detected by enzyme linked immunosorbent assay （ELISA）.Results rhTNF-α can significantly inhibit the growth of SiHa human cervical cancer cells with obvious amount-effect relationship and time-effect relationship;and the level of TP increased gradually with the increase of the level of rhTNF-α,the difference was statistically significant （F =26.87,P 〈0.01）;rhTNF-α and Capecitabine had obvious synergy,and had a dosage-dependent relationship,there was a significantly difference between the groups which received medication and control group （F =30.56,P 〈 0.05）.Conclusions rhTNF-α could actively suppress SiHa cells proliferation in vitro,and can cause a concertration-dependent increase in the level of TP,and enhance anticancer effect of Capecitabine on cervical cancer cells.

关 键 词：重组人肿瘤坏死因子-Α SIHA细胞 胸苷磷酸化酶 卡培他滨 

分 类 号：R737.33[医药卫生—肿瘤]