Cidea和Cidec促进肝脏中脂类的积累  

Cidea and Cidec Promote the Lipid Storage in Liver

作　　者：赵东宇 郁苗[1] 汪文敏 任豪 廖榕玉 徐俐[1] 周林康[1] ZHAO Dong-yu, YU Miao, WANG Wen-rain, REN Hao, LIAO Rong-yu, XU-Li, ZHOU Lin-kang(School of Life Sciences, Tsinghua University, Beijing, 100084, Chin)

机构地区：[1]清华大学生命科学学院,北京100084

出　　处：《现代生物医学进展》2018年第5期801-806,共6页Progress in Modern Biomedicine

基　　金：中国博士后科学基金项目(2013T60103;2012M520249);周林康博士受中国科学2016-2018年度青年人才托举计划的支持

摘　　要：目的:探讨Cidea和Cidec对肝脏中脂滴大小和脂类积累的影响。方法:首先,检测ob/ob肥胖小鼠的脂肪肝中Cidea和Cidec的表达情况。然后,采用腺病毒系统在野生型小鼠肝脏中过表达Cidea和Cidec,以及在ob/ob小鼠肝脏中基因沉默Cidea和Cidec,检测肝脏中脂滴大小和脂积累情况。结果:Cidea和Cidec在脂肪性肝脏中高表达。肝脏细胞中过表达Cidea和Cidec促进大脂滴的形成并能促进小鼠肝脏中的脂积累,且二者有协同作用。在脂肪肝中沉默Cidea和Cidec能缓解脂肪肝的程度,且脂合成基因的下调,线粒体活性增加。Cidea和Cidec的共沉默能进一步降低肝脏中的脂类积累。结论:Cidea和Cidec在促进肝脏的脂积累中起重要作用,并且二者有协同作用。Objective： To investigate the role of Cidea and Cidec in controlling the lipid droplets size and the lipid storage in liver. Mothods： The expressions of Cidea and Cidec in the liver of wild-type and ob/ob mouse were checked. Cidea and Cidec were overex- pressed in the wild-type hepatocytes or knocked down in ob/ob mouse hepatocytes using adenovirus mediated system. The lipid droplets size and lipid level were checked. Results： The expressions of Cidea and Cidec were dramatically increased in the liver of ob/ob mice. Overexpression of Cidea and Cidec led to the formation of large lipid droplets in hepatocytes, accompanied by significantly increased lipid storage. Knockdown of Cidea and Cidec alleviated fatty liver of ob/ob mice with dowregulated FAS and upregnlated of CPT1. Dou- ble knockdown of Cidea and Cidec led to further reduced lipid storage. Conclusion： Cidea and Cidec had synergism effect in promoting the lipid storage in liver.

关 键 词：脂肪肝 脂滴 Cidea Cidee 

分 类 号：R-33[医药卫生] R575.5