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作 者:张凡 李卫红 李峰 刘燕 于雪 王旭 高誉珊 胡艳红 张炜悦 孙天石 张楚焌 董一昕 ZHANG Fan, LI Wei-hong, LI Fen, LIU Yah, YU Xue, WANG Xu, GA O Yu-shan, HU Yan-hong, ZHANG Wei-yue, SUN Tian-shi, ZHANG Chu-jun, DONG Yi-xin(TCM College of Beijing University of Chinese Medicine, Beijing, 100029, Chin)
出 处:《现代生物医学进展》2018年第5期807-811,共5页Progress in Modern Biomedicine
基 金:国家自然科学基金项目(81273885);北京中医药大学"航海中医药"协同创新项目(522/0100604299);北京中医药大学"重点学科"开放课题--"航海中医药学"(522/0100604054)
摘 要:目的:观察中药复方天香胶囊对晕动病模型大鼠行为学与前庭核c-fos蛋白表达的影响。方法:选择雄性SD大鼠并将其随机分为正常组、模型组、阳性药组、天香胶囊低、中、高剂量组。灌胃预给药3天后,采用双轴旋转刺激法建立大鼠晕动病模型,通过晕反应指数评分、自发活动实验、异嗜高岭土实验检测各组大鼠行为学变化,Western blotting法与免疫组化法检测各组大鼠前庭核c-fos蛋白的表达。结果:与正常组大鼠相比,模型组大鼠晕反应指数和高岭土摄入量均显著增高,自发活动总路程与次数均显著减少,前庭核c-fos蛋白表达水平显著增高;与模型组相比,天香胶囊可显著降低晕动病模型大鼠晕反应指数与高岭土摄入量,提高自发活动总路程与活动次数,降低大鼠前庭核组织c-fos蛋白表达。结论:天香胶囊可有效改善晕动病模型大鼠的行为学变化,其作用机制可能与下调前庭核c-fos表达有关,提示降低前庭核兴奋性可能是天香胶囊抗晕动病的内在机制之一。Objective: To investigate the effects of Tianxiang capsule on the behavior and c-los expression in the vestibule nucleus of rats with motion sickness. Methods: Male SD rats were randomly divided into six groups, including normal group, model group, scopolamine group, low-dose, mid-dose and high-dose Tianxiang capsule groups. Prior to biaxial rotation stimulus inducing rats motion sickness model, the drugs were intragastrically administrated for 3 days. The behavioral changes of rats were evaluated by the motion sickness index, the spontaneous activity test and Kaolin pica. Meanwhile, the expression of c-los was detected by Western Blotting and Immunohistochemistty. Results: Compared with the normal group, the motion sickness index and Kaolin intake were increased, total traveling distance and frequency were decreased, the c-fos expression was up-regulated significantly in the model group. Compared with the model group, the motion sickness index and Kaolin intake were decreased, total traveling distance and frequency were increased, the c-fos expression was down-regulated significantly in Tianxiang capsule groups. Conclusions: Tianxiang capsule could effectively im- prove the behavioral changes of rats with motion sickness, which might be related to the down-regulation of c-fos in vestibular nucleus. Inhibiting the excitability of vestibular nucleus may be the pharmacological mechanisms of Tianxiang capsule in motion sickness.
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