天麻首乌片中挥发油的提取及包合工艺研究  

Study on extraction and clathration process of volatile oil in Tianma-Shouwu tablet

作　　者：苏文俏 李跃辉[2] 王银[2] 朱立华 彭艳梅[3] Su Wenqiao, Li Yuehui, Wang Yin, Zhu Lihua, Peng Yanmei(Hunan University of Chinese Medicine, Changsha 410013, Chin)

机构地区：[1]湖南中医药大学,长沙410013 [2]湖南省中医药研究院杨永华名老中医药专家传承工作室,长沙410013 [3]湖南省中医药研究院中药制剂室,长沙410013 [4]湖南国华制药有限公司,长沙410013

出　　处：《国际中医中药杂志》2018年第4期357-362,共6页International Journal of Traditional Chinese Medicine

基　　金：湖南省科学技术厅科技计划专项（2014SK4038）

摘　　要：目的 优选天麻首乌片中挥发油的提取和b-环糊精包合工艺。方法 以挥发油提取量为评价指标，采用L9（3^4）正交试验优选挥发油提取工艺；采用饱和水溶液法制备包合物，以挥发油包合率和利用率的综合评分为评价指标，采用星点设计-效应面法考察b-环糊精与挥发油的投料比、包合温度及包合时间对包合工艺的影响，对结果进行二项式方程拟合，筛选出最佳包合工艺。结果 天麻首乌片中挥发油提取最佳工艺条件为：加8倍量水，浸泡30 min，水蒸气蒸馏提取6 h；包合最佳工艺条件为：b-环糊精与挥发油的比例为9.50︰1，包合温度为43 ℃，包合时间为4.2 h。结论 优选的提取与包合工艺合理可行、稳定，重复性好。Objective To optimize the extraction and b-cyclodextrin clathration process of volatile oil in Tianma-Shouwu tablet. Methods The extraction of volatile oil was optimized through the orthogonal design L9（3^4） with the volatile oil extraction amount as assessment index. The saturated aqueous solution preparation of clathrate form was used. The volume of b-cyclodextrin and volatile oil, clathration temperature, clathration time was investigated by central composite design-response surface methodology, with the score of volatile oil inclusion ratio and utilization ratio as the index. The results were fitted by second-order polynomial equation, and then the best clathration process was determined. Results The optimization extraction process of volatile oil in Tianma-Shouwu tablet was as follows： to add 8 times water, immerse 30 minutes, steam distill 6 hours; and the best clathration process was as follows： the ratio of b-cyclodextrin and volatile oil was 9.50︰1, clathration temperature 43 ℃, and clathration time 4.2 hours. Conclusions The optimized extraction and clathration process were reasonable, stabled and repeatable.

关 键 词：挥发油类 天麻首乌片 正交试验 Β-环糊精 星点设计 

分 类 号：TQ461[化学工程—制药化工]