人血白蛋白增强顺铂对MCF7细胞的耐药作用  

Human Albumin Enhances Cisplatin Resistance to MCF7 Cells

作　　者：谭波宇[1] 韦鸿雁[1] 陈丽[1] 刘文[1] 邓楠[1] TAN Bo-vu, WEI Hone-van, CHEN Li,et al.(The First Affiliated Hospital of Hunan Normal University,Hunan Provincial People＇s Hospital, Changsha 410002, China)

机构地区：[1]湖南师范大学附属第一医院,湖南省人民医院药学部

出　　处：《中国肿瘤》2018年第4期316-320,共5页China Cancer

基　　金：湖南省药学会科研资助项目生物专项(xy2015007)

摘　　要：[目的]探讨人血清白蛋白（HSA）联用顺铂（DDP）对MCF7细胞耐药的影响。[方法]不同浓度DDP干预MCF7细胞72h,CCK8检验细胞增殖抑制率。选用DDP的IC50浓度分别与10、20μmol/L HSA联用,进一步检测联合用药对MCF7细胞的增殖抑制率。样品分为空白对照组、顺铂组、HSA组、顺铂联合HSA治疗组（10或20μmol/L）。各组处理MCF7细胞72h,实时荧光定量PCR（q RT-PCR）和免疫印迹法检测ERCC1 mRNA和蛋白表达。[结果]DDP联用10或20μmol/L HSA对MCF7的增殖抑制率分别为（50.97±1.50）%、（45.15±2.10）%,单独用药组DDP对MCF7的增殖抑制率为（55.60±5.20）%。q RT-PCR和Western blot检测表明DDP联用HSA（20μmol/L）显著增强ERCC1 m RNA和蛋白的表达。[结论 ]DDP联合使用高浓度HSA可通过上调ERCC1表达诱导化疗药物耐药。[Purpose] To investigate the effect of human serum albumin（HSA）combined with cisplatin（DDP）on the resistance of MCF7 cells. [Methods] MCF7 cells were intervened with DDP at different concentrations,and the inhibition rate of cell proliferation was examined by CCK8 after72 h treatment. The IC50 concentration of DDP was selected to be combined with 10 or 20μmol/L HSA respectively,and the inhibition rate of MCF7 cell proliferation by combined administration was further detected. The samples were divided into blank control group,DDP group,HSA group,DDP combined with HAS（10 or 20μmol/L）treatment group. Each group treated MCF7 cells for72 h and the expression of ERCC1 were detected by real-time quantitative PCR（q RT-PCR）and Western blot. [Results] DDP combined with 10 or 20 umol/L HSA on MCF7 proliferation inhibition rate were（50.97±1.50）% and（45.15±2.10）% respectively. The inhibition rate of DDP alone was（55.60±5.20）%. q RT-PCR and Western blot assays showed that DDP combined with HSA（20μmol/L）significantly enhanced ERCC1 m RNA and protein expression. [Conclusion] DDP combined with high concentration of HSA can induce resistance to chemotherapeutic drugs by upregulation of ERCC1 expression.

关 键 词：人血清白蛋白 顺铂 耐药 MCF7 ERCC1 

分 类 号：R73[医药卫生—肿瘤]