前列腺癌与良性前列腺增生组织中细胞凋亡与增殖因子表达的组织芯片检测  

Expression of apoptotic and proliferative factors in prostate cancer and benign prostatic hyperplasia using tissue microarrays

作　　者：马丁 尚吉文 王靖宇 程伟 张雁钢 MA Ding;SHANG Jiwen;WANG Jingyu;CHENG Wei;ZHANG Yangang.(Department of Urology, Shanxi Dayi Hospital, Taiyuan, 030031, China)

机构地区：[1]山西大医院泌尿外科,太原030031

出　　处：《临床泌尿外科杂志》2018年第3期224-228,共5页Journal of Clinical Urology

摘　　要：目的:许多分子标记物的表达异常与前列腺癌(PCa)发生密切相关,本次研究观察了细胞色素C(cytochrome c)、凋亡蛋白caspase-3、p21、p27及增殖细胞核抗原(PCNA)在PCa、癌旁前列腺增生(BPH)组织及BPH组织中的表达,并分析与PCa临床进展危险因素之间的相关性。方法:分别收集PCa组织标本32例和BPH组织标本64例,记录PCa患者的血清PSA水平、Gleason评分及病理学TNM分期。采用免疫组化ABC法对cytochrome c、凋亡蛋白caspase-3、p21、p27及PCNA的表达水平进行检测,并对不同分子标记物与PCa临床进展危险因素之间的相关性进行分析。结果:与BPH组织相比,cytochrome c和caspase-3在PCa组织中的表达水平增高(P<0.01),p21在PCa和BPH组织中的表达差异无统计学意义(P>0.05),p27在癌旁BPH组织中的表达显著增高(P<0.01),PCNA在PCa及癌旁BPH组织中的表达显著增高(P<0.01,P<0.05)。cytochrome c和caspase-3表达与PCa患者的Gleason评分及病理学TNM分期之间呈负相关(P<0.05)。p21和p27表达与Gleason评分之间呈负相关(P<0.05),然而PCNA表达与Gleason评分、血清PSA水平呈正相关(P<0.05)。结论:通过组织芯片技术,研究细胞凋亡与增殖因子在PCa组织中的表达对进一步明确PCa发生及临床进展的分子机制具有重要作用。Objective： To investigate the expression profiles of cytochrome c, caspase-3, p21, p27 and prolif- erating cell nuclear antigen （PCNA） in prostate cancer （PCa）, cancer-adjacent benign tissue and benign prostate hyperplasia （BPH） to determine correlations between expression profiles and clinical parameters in PCa samples. Method： Tissue specimen and clinical parameters from 32 PCa patients and 64 BPH patients were obtained. All tissue samples were processed and transferred to tissue microarrays where the expression of cytochrome c, caspase-3, p21, p27 and PCNA was immunohistochemically evaluated. Expression profiles were compared across samples and correlations with clinical parameters evaluated for PCa samples. Result： Cytochrome c and caspase-3 expression was higher in PCa cells （P〈0.01）, while p21 expression showed little difference among all prostate samples （P〉0.05）. The expression of p27 was higher in cancer-adjacent cells （P〈0.01） while PCNA expression was higher in both PCa cells and cancer-adjacent cells relative to BPH （P〈0.01, P〈0.05）. PCa correlative anal- ysis showed that cytochrome c and caspase-3 were negatively correlated with Gleason score and TNM stage （P〈0.05）, while p21 and p27 expression was negatively correlated with Gleason score （P〈0.05） and PCNA expres sion was positively correlated with Gleason score and serum PSA （P〈0.05）. Conclusion： The combined effect of cytochrome c, caspase-3, p21, p27 and PCNA plays a critical role in PCa pathogenesis facilitating further therapeutic development.

关 键 词：前列腺癌 良性前列腺增生 组织芯片技术 细胞凋亡和增殖 

分 类 号：R737.25[医药卫生—肿瘤]