脾不统血证相关疾病与血液神经递质变化关系研究  

Study on Relationship Between Spleen Failing to Control Blood Related Diseases and Blood Neurotransmitter Changes

作　　者：陈科 戴欣媛 赵宁[2] 马薇[2] 张雅月[2] 高宠[2] 陈信义[2] CHEN Ke;DAI Xinyuan;ZHAO Ning;MA Wei;ZHANG Yayue;GAO Chong;CHEN Xinyi(Shunyi Hospital of Beijing Hospital of TCM, Beijing 100700, China;Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijingl00700, China)

机构地区：[1]北京中医医院顺义医院,北京101300 [2]北京中医药大学东直门医院,北京100700

出　　处：《辽宁中医杂志》2018年第2期245-248,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(“973”计划)资助项目(2013CB531705)

摘　　要：目的：比较血液神经递质在脾不统血证的不同疾病中表达差异,探讨脾主统血理论的物质基础。方法：抽取符合纳入标准的ITP、功能失调性子宫出血、消化道肿瘤患者,并选择健康人为对照,分别空腹抽取静脉血,ELISA法检测血液神经递质表达水平。结果：4组组间比较5-HT、VIP、β-EP均具有统计学意义（P〈0.05）;与正常人比较,功能失调性子宫出血、消化道肿瘤及ITP患者5-HT、VIP、β-EP检测值均较下调;但ITP患者5-HT检测值较其他三组显著上调,而VIP检测值较正常人明显下调。组间两两比较,消化道肿瘤患者与正常人比较,5-HT、VIP、β-EP检测值下调明显（P〈0.05）;功能失调性子宫出血患者与消化道肿瘤患者比较,VIP、β-EP检测值下调明显（P〈0.05）。ITP患者与正常人、功能失调性子宫出血患者及消化道肿瘤患者比较,5-HT、VIP检测值均下调明显（P〈0.05）。β-EP检测值比较中,ITP患者较功能失调性子宫出血患者有所下调（P〈0.05）。结论：5-HT、VIP、β-EP含量与＂脾主统血＂理论存在一定关联,但不同疾病间5-HT、VIP、β-EP含量改变存在差异。Objective： To figure out the biological mechanism of the theory‘spleen controlling the blood＇based on the comparison on expression levels of blood neurotransmitters in different diseases. Methods： Fasting vein blood was withdrawn from the patients with ITP,DUB and digestive cancer. The samples from healthy subjects were obtained as a control group. ELISA was applied to detect the expression levels of blood neurotransmitters. Results： Statistically significant difference was found among four groups＇ 5-HT,VIP and β-EP（P〈 0. 05）. The levels of 5-HT,VIP and β-EP in three patient groups were remarkably lower than those in the control groups. However,a higher 5-HT level and a lower VIP level were found in ITP group,in comparison with the other 3 groups. 5-HT,VIP and β-EP levelsof patients were lower than those in control group（P〈 0. 05）. VIP and β-EP levelsof DUB patients were lower than those in digestive cancer group（P〈 0. 05）. 5-HT and VIP levels in ITP group were lower than that those in the other 3 groups（P〈 0. 05）. β-EP level in ITP group was lower than that in DUB group（P〈 0. 05）. Conclusion： 5-HT,VIP and β-EP levels are related to the theory of spleen controlling blood. The changes in 5-HT,VIP and β-EP levels vary in different diseases.

关 键 词：脾气虚证 血管活性肠肽 Β-内啡肽 5-羟色胺 

分 类 号：R735[医药卫生—肿瘤]