白藜芦醇通过SRIT1/NOX-p47信号通路抑制高糖诱导的大鼠胸主动脉平滑肌细胞增殖  

Resveratrol inhibits high glucose-induced rat thoracic aorta smooth muscle cells proliferation by activating SRIT1/NOX-p47 signaling pathways

作　　者：吴艳宁 赵占胜[1] 王婷[1] 张甜甜[3] 底孟林茜 金玉怀[5] WU Yan-Ning;ZHAO Zhan-Sheng;WANG Ting;ZHANG Tian-Tian;DIMENG Lin-Qian;JIN Yu-Huai(Department of Endocrinology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, Chin)

机构地区：[1]河北医科大学第二医院内分泌科 [2]河北医科大学第二医院体检中心,河北省石家庄市050000 [3]山东省寿光市人民医院内分泌科,山东省寿光市262700 [4]华北理工大学临床医学系,河北省唐山市063009 [5]河北医科大学病原生物学教研室,河北省石家庄市050017

出　　处：《中国动脉硬化杂志》2018年第3期232-236,共5页Chinese Journal of Arteriosclerosis

基　　金：河北省科技计划项目(10246430D)

摘　　要：目的研究白藜芦醇（Res）对高浓度葡萄糖孵育下的血管平滑肌细胞（VSMC）SRIT1及NADPH氧化酶-p47（NOX-p47）蛋白表达的影响,探究白藜芦醇对高糖诱导的血管平滑肌细胞增殖抑制作用的机制。方法以大鼠胸主动脉平滑肌细胞株A7r5为研究对象,采用MTT法检测细胞增殖活性;流式细胞术检测VSMC细胞周期进程;采用Western blot技术检测SIRT1、NOX-p47蛋白的表达。结果高浓度葡萄糖（25 mmol/L）培养可明显促进VSMC增殖,诱导G0/G1期细胞向S期转化,明显增加NOX-p47蛋白表达,减少SIRT1蛋白表达;白藜芦醇（25、50、100μmol/L）以浓度依赖形式抑制高糖孵育下VSMC增殖活性,阻止其由G0/G1期向S期转变,降低VSMC NOXp47表达,增加SIRT1蛋白表达。结论白藜芦醇可能通过SIRT1/NOX-p47蛋白信号通路干预高糖诱导的VSMC增殖,对2型糖尿病的大血管病变起到防治作用。Aim To investigate the effects of resveratrol（ Res） on SRIT1 and NADPH oxidase-p47 protein（ NOX-p47） expressions,and mechanisms of Res inhibiting rat thoracic aorta smooth muscle cells proliferation induced by high glucose. Methods Vascular smooth muscle cell（ VSMC） lines A7 r5 were used. VSMC proliferation was evaluated by MTT assay and cell counting. The cell cycle was examined by flow cytometry. The SRIT1 and NOX-p47 protein expressions were also evaluated by Western blot. Results High glucose increased cell proliferation in rat VSMC,induced cell transition from the G0/G1 phase to the S phase,increased NOX-p47 protein expression and decreased SRIT1 expression. Res（ 25,50,100 μmol/L） significantly inhibited transition of cultured VSMC from G0/G1 phase to the S phases in a concentration-dependent manner,up-regulated SIRT1 expression and decreased expression of NOX-p47 protein induced by high glucose. Conclusion Under high glucose concentrations,Res significantly inhibited VSMC proliferation by arresting cell cycle by down-regulating the protein expression of NOX-p47 and up-regulating the SIRT1 protein expression,suggesting its protective role against type 2 diabetic macrovascular disease.

关 键 词：白藜芦醇 高浓度葡萄糖 SIRT1 NADPH氧化酶-p47 细胞增殖 血管平滑肌细胞 

分 类 号：R363[医药卫生—病理学]