Large-scale functionalization of biomedical porous titanium scaffolds surface with TiO_2 nanostructures  

医用多孔钛支架表面功能化二氧化钛纳米结构构建的研究（英文）

作　　者：Lei Qian Peng Yu Jinquan Zeng Zhifeng Shi Qiyou Wang Guoxin Tan Chengyun Ning 钱磊;于鹏;曾锦全;石志锋;王其友;谭帼馨;宁成云(School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China;Biomedical Engineering Key Laboratory of Guangdong Province, Guangzhou 510641, China;Guangzhou Tieyi Middle School, Guangzhou 510660, China;Department of Spine Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China)

机构地区：[1]School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,China [2]Biomedical Engineering Key Laboratory of Guangdong Province,Guangzhou 510641,China [3]Guangzhou Tieyi Middle School,Guangzhou 510660,China [4]Department of Spine Surgery,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China [5]School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China

出　　处：《Science China Materials》2018年第4期557-564,共8页中国科学（材料科学（英文版）

基　　金：supported by the National High Technology Research and Development Program of China(2015AA033502);the National Natural Science Foundation of China(51372087,51232002 and 51541201);the Science and Technology Planning Project of Guangdong Province,China(2014A010105048);the Natural Science Foundation of Guangdong Province(2015A030313493 and 2016A030308014);the State Key Laboratory for Mechanical Behavior of Materials,China(20141607);the Technological Projects of Guangzhou,China(201604020110)

摘　　要：Construction of functional porous titanium scaffold is drawing ever growing attention, due to its effectiveness in solving the mechanical mismatch between titanium implant and bone tissue. However, the poor water permeability as well as the problem in achieving uniform surface modification inside scaffold hinders the further biomedical application of porous titanium scaffold. In this study, largescale functional Ti O2 nanostructures（nanonetwork, nanoplate and nanowire） were constructed on three-dimensional porous titanium scaffolds surface via an effective hydrothermal treatment method. These nanostructures increase the hydrophilicity of the titanium scaffold surface, facilitating the cell culture medium to penetrate into the inner pore of the scaffold. Zeta potential analyses indicate that the surface electrical properties depend on the nanostructure, with nanowire exhibiting the lowest potential at p H 7.4. The influence of the nano-functionalized scaffold on protein adsorption and cell adhesion was examined. The results indicate that the nano-functionalized surface could modulate protein adsorption and bone marrow derived mesenchymal stem cells（BMSCs） adhesion, with the nanowire functionalized porous scaffold homogeneously promoting protein adsorption and BMSCs adhesion. Our research will facilitate future research on the development of novel functional porous scaffold.由于可有效解决钛植入体与骨组织在力学方面的不匹配性,功能化多孔钛支架的研发已得到广泛关注.然而,多孔钛支架往往液体渗透性差且难以实现大面积均匀的表面处理,极大阻碍了多孔支架用于临床骨缺损修复治疗.本研究采用高效水热处理方法在三维多孔钛支架内外表面构建均匀二氧化钛纳米结构(纳米网络结构、纳米片结构和纳米线结构).这些纳米结构增强了多孔钛支架的亲水性,利于细胞培养液进入到多孔支架孔内.固体表面Zeta电位分析表明材料表面电势依赖于材料表面纳米结构,其中在p H为7.4条件下纳米线结构具有最低的表面电势.纳米功能化的多孔支架具有调控蛋白吸附和骨髓间充质干细胞黏附的作用,其中纳米线结构功能化的多孔钛支架可同时促进蛋白吸附和细胞黏附铺展.该研究工作将对新型功能化医用多孔支架的研发具有指导意义.

关 键 词：porous titanium HYDROTHERMAL NANOSTRUCTURE SCAFFOLD cell adhesion 

分 类 号：R318.08[医药卫生—生物医学工程]