CDH11甲基化在宫颈癌中的表达及临床意义  

Expression and clinical significance of CDH11 gene methylation in cervical cancer

作　　者：姚霁航[1] 杨紫伟[1] 郭科军[1] Yao Jihang;Yang Ziwei;Guo Kejun(Department of Gynecology, The First Affiliated Hospital of China Medical University,Liaoning Shenyang 110000, China.)

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110000

出　　处：《现代肿瘤医学》2018年第10期1581-1584,共4页Journal of Modern Oncology

摘　　要：目的:探讨钙黏附蛋白11(cadherin-11,CDH11)甲基化在宫颈癌中的表达及临床意义。方法:应用甲基化特异性聚合酶链反应检测CDH11基因在3株宫颈癌细胞系、1株人表皮永生化细胞株、30例宫颈癌组织、17例正常宫颈组织中的甲基化状态。统计患者临床资料,分析甲基化同临床病理特征之间的联系。结果:CDH11在3株宫颈癌细胞系中,均出现不同程度甲基化;在1株人表皮永生化细胞株中未检测到甲基化。CDH11在宫颈癌组织中的甲基化率为56.7%(17/30),显著高于正常宫颈组织23.5%(4/17),差异具有统计学意义(P<0.05)。CDH11基因甲基化在不同分期的宫颈癌组织中检出率无统计学意义(P>0.05);在不同年龄段、是否有淋巴结转移以及分化级别上,CDH11基因的甲基化率差异无统计学意义(P>0.05)。结论:宫颈癌组织中CDH11基因甲基化水平高于正常宫颈组织,差异有统计学意义。CDH11基因甲基化可能是宫颈癌独特的基因甲基化谱成员之一,可以成为宫颈癌早期诊断的新的标志物。Objective：To investigate the expression and clinical significance of CDH11 gene methylation in cervical cancer.Methods：CDH11 methylation was detected by methylation specific PCR（MSP）in 3 cervical cancer cell lines,1 normal human immortalized keratinocytes cell line,30 cases of cervical cancer fresh specimens and 17 normal cervical tissues.Patients＇ clinicopathological features were collected and analyzed.Results：MSP revealed that CDH11 gene methylation founded in 3 cervical cancer cell lines,and in 56.7% of cervical cancer patients 4 of 17（23.5%）normal cervical tissues showed methylation.No methylation was detected in HacaT cell line and normal cervical tissues.The methylation of CDH11 was not associated with age,FIGO stage,lymph node metastesis and classification.Conclusion：The CDH11 promoter methylation which occurs in cervical cancer was significantly higher than the normal controls.Our results indicated that CDH11 gene methylation as a frequently event involving in the tumorigenesis of cervical cancer,and may be expected to be a new marker to predict the occurrence of patients with cervical cancer.

关 键 词：宫颈癌 CDH11 甲基化 甲基化特异性聚合酶链反应 

分 类 号：R737.3[医药卫生—肿瘤]