STZ致高血糖对成年大鼠精囊组织结构影响的定量研究  

A quantitative study of the effect of STZ-induced hyperglycemia on histological structures of seminal vesicles in mature rats

作　　者：李雨根[1] 李进铭 马利[2] 郭洋[3] 邓显忠[1] 杨正伟[3] LI Yugen;LI Jinming;DENG Xianzhong;MA Li;GUO Yang;YANG Zhengwei(Department of Urological Surgery, Affiliated Hospital of North Sichuan Medical College;Department of Histology and Embryology;Morphometric Research Laboratory, North Sichuan Medical College, Nanchong 637000, China)

机构地区：[1]川北医学院附属医院泌尿外科,南充637000 [2]川北医学院组胚教研室,南充637000 [3]川北医学院形态定量研究室,南充637000

出　　处：《中国体视学与图像分析》2018年第1期74-79,共6页Chinese Journal of Stereology and Image Analysis

基　　金：四川省教育厅科研项目(15ZB0189)

摘　　要：目的定量研究高血糖对精囊组织结构的影响。方法给成年雄性Sprague-Dawley大鼠腹腔内注射链脲佐菌素(STZ)或赋形剂,30天后取一侧精囊制作甲基丙烯酸树脂包埋切片,光镜下用体视学方法估计精囊内各种主要结构的总体积。结果 STZ导致9只动物高血糖,其中8只动物的精囊有明显的病理学损害:与对照动物相比,腺腔变得狭小,腺上皮皱缩,2只动物还见明显的炎性细胞浸润;整个精囊的体积减少77.9%,其腺腔、腺上皮、固有层、肌层和外膜(其他组织)的总体积分别减少95.9%、74.5%、75.1%、39.4%和37.0%。结论 STZ诱发的高血糖可导致大鼠精囊主要结构的严重萎缩,尤其是腺腔和黏膜的萎缩。Objective To quantitatively study the effect of hyperglycemia on tissue structures in the seminal vesicle. Methods Mature male Sprague-Dawley rats were given an intraperitoneal injection of streptozotocin（STZ） or vehicle. 30 days later,unilateral seminal vesicles were removed,glycol methacrylate resin-embedded sections prepared and the total volumes of various key structures in the seminal vesicle estimated using stereological methods under a light microscope. Results STZ resulted in hyperglycemiain 9 animals and 8 of them had marked pathological damage in the seminal vesicle： in comparison with control animals,the glandular cavity became narrow and small and the glandular epithelium shrank; apparent inflammatory cells infiltration was observed in 2 animals; the volume of the entire seminal vesicle decreased by 77. 9%,and the total volumes of the glandular lumen,epithelium,lamina propria,muscularis and adventitia（other tissue） reduced by 95. 9%,74. 5%,75. 1%,39. 4% and 37. 0%,respectively. Conclusions STZ-induced hyperglycemia would bring about severe atrophy of main structures in the seminal vesicle,especially atrophy of the glandular lumen and mucosa.

关 键 词：大鼠 链脲佐菌素 高血糖 精囊 组织学 体视学 

分 类 号：R361[医药卫生—病理学] R587.1[医药卫生—基础医学]