细胞代谢组学样品前处理研究进展  

The Research Development of Sample Pretreatment in Cell Metabolomics

作　　者：徐佳 刘其南[2] 翟园园[2] 单进军 姚卫峰 张丽 Xu Jia;Liu Qinan;Zhai Yuanyuan;Shan Jinjun;Yao Weifeng;Zhang Li(Jiangsu Key Laboratory ofPediatrtc Respzratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China)

机构地区：[1]南京中医药大学江苏省儿童呼吸疾病(中医药)重点实验室,南京210023 [2]南京中医药大学江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心,南京210023

出　　处：《中国细胞生物学学报》2018年第3期418-425,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金面上项目(批准号:81573554);江苏高校"青蓝工程"和江苏省"六大人才高峰"高层次人才项目(批准号:2016-YY026)资助的课题~~

摘　　要：细胞代谢组学是系统生物学的重要组成部分,是对生物系统进行整体和动态的认识的科学,主要进行小分子代谢物定性和定量分析研究,观察代谢物的浓度变化,从而在细胞水平上考察代谢机制。细胞代谢组学的工作流程包括:实验设计、样品采集、样品处理、代谢物分析和数据处理。其中,样品前处理方法不尽相同,而设计一个合理方便的样品前处理方法对后期开展代谢组学至关重要。现简要综述现阶段对细胞代谢组学样品前处理的研究成果和常用方法。Cell metabolomics is an important part of systemic biology and a scientific for the overall and dynamic understanding of biological systems. It mainly studies the qualitative and quantitative analyses of small molecular rnetabolites and observes the changes of metabolites to investigate the metabolic mechanism. Cell metabolomics workflow includes experimental design, sampling, sample processing, metabolite analysis, and data processing. The pretreatment methods of cell metabolomics are not the same, and the design of a reasonable and convenient sample pretreatment method for the late development of metabolomics is essential. We present a brief review of the results of the sample pretreatment at this stage and the the method commonly used in cellular metabolomics samples.

关 键 词：细胞代谢组学 样品前处理 淬灭 提取 归一化 

分 类 号：Q2-33[生物学—细胞生物学]