miRNA-27a靶向SPRY2调控Wnt/β-catenin信号通路对人卵巢癌细胞增殖和侵袭能力的影响  

Effect of miRNA-27a targeted regulation of SPRY2 for Wnt/β-catenin signaling pathway on the proliferation and invasion ability of human ovarian cancer cells

作　　者：刘卫民[1] 柳学芳[1] 龚文端 LIU Weimin;LIU Xuefang;GONG Wenduan(Department of Obstetrics and Gynecology, Central Hospital of Qianjiang, Qianjiang 433100, Hubei, China)

机构地区：[1]潜江市中心医院妇产科,湖北潜江4331000

出　　处：《癌症进展》2018年第3期282-285,共4页Oncology Progress

摘　　要：目的探讨mi RNA-27a靶向SPRY2调控Wnt/β-catenin信号通路对人卵巢癌细胞增殖和侵袭能力的影响。方法采用荧光定量RT-PCR和Western blot法检测卵巢癌组织、癌旁正常上皮结缔组织和HO-8910细胞、正常卵巢上皮细胞的mi RNA-27a和SPRY2表达水平;MTT法检测转染细胞的增殖情况;Transwell实验检测细胞侵袭情况;荧光素酶分析验证mi RNA-27a对SPRY2的靶向调控能力;免疫荧光染色检测mi RNA-27a与SPRY2对Wnt/β-catenin信号通路的调控能力。结果卵巢癌组织、HO-8910细胞中mi RNA-27a的表达水平分别高于癌旁正常上皮结缔组织、卵巢正常上皮细胞(P﹤0.05),而SPRY2的m RNA和蛋白表达水平均低于癌旁正常组织及卵巢正常上皮细胞(P﹤0.05)。与B组相比,C组和E组HO-8910细胞增殖及侵袭能力均下调(P﹤0.05)。F组的荧光素酶活性明显高于G组(P﹤0.01)。C组的HO-8910细胞中,β-catenin表达上升,且进入细胞核。结论 mi RNA-27a通过靶向抑制SPRY2激活Wnt/β-catenin信号通路,从而促进人卵巢癌细胞的增殖和侵袭能力。Objective To explore the effect of mi RNA-27 a targeted regulation of SPRY2 for Wnt/β-catenin signaling pathway on the proliferation and invasi on ability of human ovarian cancer cells. Method Fluorescence quantitative RT-PCR and Western blot were used to detect the level of mi RNA-27 a and SPRY2 in ovarian cancer tissues and adjacent normal tissues as well as in cell line HO-8910. MTT was applied to detect the proliferation of transfected cells. Cell invasion ability was measured using Transwell assay. The luciferase analysis was utilized to verify the targeted regulation of mi RNA-27 a on SPRY2. Immunofluorescence staining test was used to detect the regulatory ability of mi RNA-27 a and SPRY2 on Wnt/β-catenin signaling pathways. Result The expression of mi RNA-27 a was higher in HO-8910 cells than in normal ovarian tissues and adjacent normal epithelial connective tissues（P〈0.05）, while the SPRY2 m RNA and protein expression were decreased in HO-8910 cells than that in adjacent normal tissues and normal ovarian epithelial cells（P〈0.05）. Compared with the B group, the proliferation and invasion ability of HO-8910 cells were decreased in C group and E group（all P〈0.05）. The luciferase activity was obviously higher in F group than that in G group（P〈0.01）. The level of β-catenin was increased in HO-8910 cells of C group, and entered the nucleus. Conclusion mi RNA-27 a inhibits SPRY2 to activate Wnt/β-catenin signaling pathway, so as to promote the proliferation and invasion ability of human ovarian cancer cell.

关 键 词：miRNA-27a SPRY2 卵巢癌 WNT/Β-CATENIN 增殖 侵袭 HO-8910 

分 类 号：R737.31[医药卫生—肿瘤]