miR-130b调控RAB34的表达在胰腺癌发生发展中的作用  

Effect of mi R-130b Targeting RAB34 Expression in Occurrence and Development of Pancreatic Cancer

作　　者：何峰 陈曦 程方雄[1] 闵小春[1] 曹忠厚 丁锋[1] HE Feng;CHEN Xi;CHENG Fangxiong;MIN Xiaochun;CAO Zhonghou;DING Feng(Department of Laboratory, Puai Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China;Clinical Laboratory, Jianghan Center for Disease Prevention and Control in Wuhan, Wuhan 430064, China;Disease Prevention and Control of Changjiang River Administration in Wuhan, Wuhan 430019. China)

机构地区：[1]华中科技大学同济医学院附属普爱医院检验科,武汉430077 [2]武汉市江汉区疾病预防控制中心检验科,武汉430064 [3]武汉市长江航务管理局疾病预防控制中心,武汉430019

出　　处：《肿瘤防治研究》2018年第3期144-147,共4页Cancer Research on Prevention and Treatment

基　　金：武汉市卫计委科研基金(WX13C15)

摘　　要：目的探讨miR-130b调控Ras相关蛋白34(Ras-related proteins in brain 34,RAB34)在胰腺癌中的表达。方法收集22例胰腺癌及其癌旁组织标本,采用免疫组织化学、Western blot和q RT-PCR法检测RAB34的表达;q RT-PCR法检测miR-130b表达;细胞转染法检测miR-130b调控RAB34表达,双荧光素酶报告基因检测系统验证miR-130b结合RAB34的3’UTR的靶位点。结果与癌旁组织对比,RAB34在胰腺癌组织中显著高表达,且miR-130b显著低表达;转染miR-130b mimics可抑制RAB34的表达;RAB34是mi R-130b作用的靶基因。结论 RAB34是胰腺癌的分子标志物,miR-130b可调控其表达。Objective To investigate the expression of Ras-related proteins in brain 34（RAB34） regulated by mi R-130 b in pancreatic cancer. Methods We collected 22 paired specimens of primary pancreatic cancer and adjacent normal tissues specimens. Immunohistochemistry, Western blot and q RT-PCR were employed to detect the expression level of RAB34. Meanwhile, the expression of mi R-130 b was detected by q RT-PCR. Cell transfection method was used to discover the regulation of mi R-130 b on RAB34 gene. The luciferase reporter assay was applied to identify whether RAB34 was directly targeted by mi R-130 b. Results Compared with adjacent tissues specimens, mi R-130 b was significantly downregulated and its target gene RAB34 was upregulated in pancreatic cancer tissues. Overexpression of mi R-130 b remarkably inhibited the expression of RAB34. Moreover, the dual luciferase assay revealed that RAB34 was directly targeted by mi R-130 b. Conclusion RAB34 might have a considerable potential in prognosis identification of pancreatic cancer and be regulated by mi R-130 b.

关 键 词：胰腺癌 RAB34 miR-130b 

分 类 号：R735.9[医药卫生—肿瘤]