可视环介导恒温扩增技术检测实验猴小肠结肠炎耶尔森菌  

Visible Loop-mediated isothermal amplificationmethod for examination of experimental monkey Yersinia enterocolitica

作　　者：温和心 龙英全 蒋荣华 盘宝进 罗广生 陈立军 WEN He-xin;LONG Ying-quan;JIANG Rong-hua;PAN Bao-jin;LUO Guang-sheng;CHEN Li-jun(Guigang Entry-exit inspection and Quarantine bureau, Guigang 537100, China;National Key Primate Laboratory, Nanning 530000,China;Yulin Entry-exit inspection and Quarantine bureau, Yulin 530002,China)

机构地区：[1]贵港出入境检验检疫局,广西贵港537100 [2]灵长类实验动物国家重点实验室,广西南宁530028 [3]玉林出入境检验检疫局,广西玉林530000

出　　处：《中国兽医杂志》2018年第1期40-42,46,I0004,共5页Chinese Journal of Veterinary Medicine

基　　金：国家质检总局科技计划项目(2016IK072)

摘　　要：本研究在传统环介导恒温扩增反应体系中引入羟基萘酚蓝指示剂,对反应体系进行高温-低温前处理和添加甜菜碱等优化措施提高试验敏感性,液面覆盖矿物油降低气溶胶污染,据此建立的猴小肠结肠炎耶尔森菌可视LAMP检测方法结果判定直观明了,敏感度为6 CFU/反应,无非异性。In this study,the indicator hydroxynaphthol blue was added to reaction system of the traditional Loop-mediated isothermal amplification. The reaction system was prepared by heating-cooling and betaine was added to the reaction system to improve the sensibility. The liquid surface was covered by mineral oil. Base on these,we built a visible loop-mediated amplification method for detecting experimental monkey Yersinia enterocolitica. The visible LAMP/s sensibility was 6 cfu/reaction. The visible LAMP showed intuitive and specific results,and the method was more efficient and had higher detection rate than germiculture.

关 键 词：可视LAMP 检测 小肠结肠炎耶尔森菌 

分 类 号：V448.251[航空宇航科学与技术—飞行器设计]