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作 者:罗旭[1] 方芳[1] 李倩倩 许腾[1] 王艳[1] 王凤忠[1] LUO Xu;FANG Fang;LI Qianqian;XU Teng;WANG Yan;WANG Fengzhong(Institute of Food Science and Technology, Chinese Academy ~ Agricultural Sciences, Beijing 100193)
机构地区:[1]中国农业科学院农产品加工研究所,北京100193
出 处:《核农学报》2018年第5期952-958,共7页Journal of Nuclear Agricultural Sciences
基 金:农业部现代农业产业技术体系项目(CARS-04);农业部"948"项目(2016-X31);中国农业科学院创新工程(125161015000150013);北京市粮经作物产业创新团队(BAIC09-2017)
摘 要:为探究大豆萌芽过程中生物活性物质及抗氧化能力变化规律,以黑河43、黔豆7号、徐豆14和中黄13大豆为研究对象,对其萌芽过程中总多酚、总黄酮、大豆异黄酮和γ-氨基丁酸(GABA)含量进行动态监测,同时评价其抗氧化能力。结果表明,大豆经萌芽处理后,GABA大量积累,4种大豆GABA含量峰值分别为萌芽前的30、37、6.8和6.5倍,黔豆7号对GABA的富集最显著;总多酚、总黄酮及异黄酮的含量均有所上升,抗氧化能力有所增强,其中,黔豆7号总黄酮和异黄酮含量最高,说明其抗氧化能力也最强。相关性分析表明,4种大豆抗氧化能力与总多酚、总黄酮、大豆异黄酮含量显著相关。综上,萌芽是提高大豆功能性组分及抗氧化能力的有效手段;黔豆7号是富集生物活性物质的最佳品种,可用于功能性产品的开发。本研究为萌芽大豆的开发利用提供了科学依据,为后续的进一步研究奠定了理论基础。The changes of total phenolic contents( TPC),total flavonoid contents( TFC),and content of isoflavone andγ-aminobutyric acid( GABA) and antioxidant activities of germinated soybeans were investigated in this paper. Four Chinese soybean cultivars,HH43,QD7,XD14 and ZH13,were evaluated. The results showed that GABA content of HH 43,QD 7,XD 14 and ZH 13 increased significantly,with 30-,37-,6. 8-,and 6. 5-fold increase,respectively,at peak level compared to ungerminated seeds,and QD 7 had the highest content of GABA. TPC,TFC and isoflavone were also enhanced when germinating,as well as the antioxidant activity. Correlation analysis showed that there was a positive correlation between antioxidant activity and content of TFC,TPC and isofalvone. QD 7 had the highest contents of TFC and isoflavone,and therefore exerted the strongest antioxidant activity. Current results suggested that germination could improve the nutritional value of soybean and that the QD 7 soybean was a cultivar which could be used to produce functional food. This study provided theoretical basis for the further research and utilization of germinated soybeans.
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