磨牙缺失对幼年大鼠海马CA1区神经元及BDNF、TrkB表达影响的研究  

Study on the effects of molar-missing on the neurons and BDNF and TrkB expression in the hippocampus CA1 region of juvenile rats

作　　者：李想 李伯琦[2] 于璐[2] 刘奕杉[2] 周晓慧 迪丽努尔.阿吉 LI Xiang;LI Boqi;YU Lu;LIU Yishan;ZHOU Xiaohui;Dilinuer. Aft(Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 83005)

机构地区：[1]新疆医科大学第一附属医院口腔修复科,乌鲁木齐830054 [2]新疆医科大学第一附属医院儿牙预防科,乌鲁木齐830054

出　　处：《现代口腔医学杂志》2018年第2期65-68,共4页Journal of Modern Stomatology

基　　金：新疆维吾尔自治区级青年科学基金(2016D01C316)

摘　　要：目的探讨磨牙缺失对幼年大鼠海马CA1区神经元及脑源性神经营养因子(BDNF)、酪氨酸蛋白激酶B受体(TrkB)表达的影响。方法 50只雄性SD大鼠,随机分为五组,每组10只,分别为正常对照组(A组)、3颗磨牙拔除组(B组)、6颗磨牙拔除组(C组)、9颗磨牙拔除组(D组)、12颗磨牙拔除组(E组),8周后处死取大鼠海马组织,分别采用HE染色法和免疫组织化学染色法,观察各组大鼠海马CA1区神经细胞结构的变化及BDNF、TrkB蛋白的表达情况。结果 (1)A组及B组海马CA1区神经组织形态结构正常,基本无病理性改变;C、D、E组出现细胞层数的减少和细胞排列稀疏、紊乱。(2)随着各组中大鼠磨牙缺失数目的增加,大鼠海马CA1区的BDNF、TrkB的表达量呈现降低趋势,与A组相比,B组BDNF、TrkB蛋白的阳性细胞表达无明显变化(P>0.05),而C、D、E组BDNF、TrkB蛋白阳性细胞表达差异有统计学意义(P<0.05)。结论较多数目的磨牙缺失可以导致幼年大鼠海马CA1区神经细胞损伤及BDNF、TrkB蛋白表达的减少。Objective To investigate the effect of molar-missing on neurons and Brain source neurotrophic factor（BDNF）and tyrosine kinase B receptor（TrkB）expression in the hippocampus CA1 region of juvenile rats. Methods50 SD male rats were randomly divided into five groups. the number of 3、6、9 and 12 molars-missing experimental control groups（B, C, D, E）no-molar-missing in normal control group（A）. Rats were sacrificed after 8 weeks of treatment and the hippocampus tissue were taken. Observe the changes of nerve cell structure and the expression of BDNF and TrkB protein in hippocampus CA1 region of each group by using He staining and immunohistochemical staining. Results（1）Group A and group B were normal in the hippocampus CA1 area and had no pathological changes. C, D, and E groups showed decreased cell number and cell lines were sparse and disordered.（2） Groups of rats with the increase of molar teeth number missing, the expression of BDNF in the rat hippocampus CA1 area and TrkB quantity showed A trend of decrease, compared with group A, group B BDNF, TrkB protein positive cells express no significant change（P〉0.05）,and C, D, E group of BDNF, TrkB protein positive cells expression difference was statistically significant（P 〈0.05）.Conclusion A large number of molar deletions can lead to neuronal damage and decrease of BDNF and TrkB protein expression in hippocampus CA1 region of juvenile rats.

关 键 词：磨牙缺失 脑源性神经营养因子 酪氨酸蛋白激酶B受体 海马组织 

分 类 号：R78[医药卫生—口腔医学]