金针菇Fv-Afe1基因的功能初探  

Preliminary Study on the Function of Fv-Afe1 Gene in Flammulina velutipes

作　　者：姜思源 丑天胜 李肖[2] 黄蓉梅 谢宝贵[1] JIANG Si-yuan;CHOU Tian-sheng;LI Xiao;HUANG Rong-mei;XIE Bao-gui(Mycological Research Center of Fujian Agricultural and Forestry University, Fuzhou 350002;Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080)

机构地区：[1]福建农林大学菌物研究中心,福州350002 [2]中国科学院微生物研究所,北京100080

出　　处：《生物技术通报》2018年第3期230-234,共5页Biotechnology Bulletin

基　　金：国家重点基础研究发展计划(2014CB138302)

摘　　要：从金针菇菌株L11基因组数据筛选出一个腺苷酸合成酶超家族基因Fv-Afe1。结合转录组数据分析基因结构,利用生物信息学软件对基因及其氨基酸序列生理生化指标进行预测,与NCBI已公布的其他物种的氨基酸序列进行系统发育分析,运用qRT-PCR技术和转录组表达谱数据对Fv-Afe1在金针菇各发育时期进行表达分析。结果显示,Fv-Afe1全长为967 bp,具有4个外显子和3个内含子,该基因氨基酸三级结构具有两个结构域,属于胞内酶,无跨膜结构,为真菌腺苷酸合成酶超家族成员;该基因在伸长期菌柄高表达,成熟期菌柄次之。According to genome data of a Flammulina velutipes strain L11,we selected Fv-Afe1 belonging to adenylate-forming enzymessuperfamily. Transcriptome of F. velutipes was used to analyze gene structure,bioinformatics software to predict the amino acid sequence of FvAfe1,the predicted results and amino acid sequences of other species in NCBI were combined to construct phylogenetic tree,and qRT-PCRand DGE（digital gene expression profiling）data to analyze the gene expression in different growth stage. The results showed Fv-Afe1 genecontained 967 bp,including 4 exons and 3 introns. The results of bioinformatics revealed that the gene had two domains at tertiary structure ofamino acids. Besides,the product of Fv-Afe1 was intracellular enzyme and no transmembrane structure,Fv-Afe1 belonging to the adenylateforming enzymes superfamily was confirmed by analyzing phylogenetic tree,expression test showed that Fv-Afe1 had the highest expression instipe-elongation stage and the secondly highest in stipe-maturation.

关 键 词：金针菇 子实体发育 腺苷酸合成酶超家族 

分 类 号：S646.15[农业科学—蔬菜学]