三氯生与镉单一及复合污染对土壤呼吸和酶活性的影响  

Effects of Single-Factor and Combined Contamination of Triclosan and Cadmium on Respiration and Enzyme Activity of Soil

作　　者：王凤花[1,2,3] 张振国 贾文 WANG Fenghua;ZHANG Zhenguo;JIA Wen(College of Resources and Environment, Shandong Agricultural University, Taian, Shandong 271018, China;National Engineering Laboratory for Efficient Utilization of Soiland Fertilizer Resources, Taian, Shandong 271018, China;Key Laboratory of Agrieultural Environment in Universities ofShandong, Taian, Shandong 271018, China;School of Economies and Management, Dalian Minzu University, Dalian, Liaoning 116650, China)

机构地区：[1]山东农业大学资源与环境学院,泰安271018 [2]土肥资源高效利用国家工程实验室,泰安271018 [3]山东省高校农业环境重点实验室,泰安271018 [4]大连民族大学经济管理学院,大连116650

出　　处：《土壤学报》2018年第2期422-431,共10页Acta Pedologica Sinica

基　　金：中国博士后基金面上项目(2016M602168);国家级大学生创新创业训练计划项目(201410434049)资助~~

摘　　要：为了解典型药品和个人护理品(PPCPs)——三氯生与镉复合污染的生态效应,采用室内培养实验和联合毒性预测模型,首次评价了三氯生与镉单一及复合污染对土壤呼吸及参与土壤碳氮循环的相关酶活性的生态毒性。结果表明:三氯生与镉单一及复合污染对土壤呼吸呈现激活—抑制—激活的生态效应;刺激了蛋白酶活性,激活率先降低后升高,56 d时达到最大。整个实验期间均抑制了蔗糖酶活性,镉(10.0 mg kg-1)单一污染培养14 d抑制率达到最大值(81%),三氯生单一胁迫呈现负的剂量效应关系,两者复合污染无显著的剂量效应关系。联合效应评价模型预测表明,相比三氯生或镉单一污染,两者的复合胁迫对土壤呼吸呈现随时间变化的拮抗—协同—加和效应,对土壤蛋白酶呈现协同—加和—协同的联合效应,而对土壤蔗糖酶活性则主要为协同效应。【Objective】Although some studies have been reported on environmental risk assessment of single-factor contamination of triclosan（TCS）, a typical pharmaceuticals and personal care products（PPCPs）, little has on effects of combined contamination of TCS and cadmium on ecosystems. The aim of this study is to evaluate comprehensively environmental risks of combined contamination of TCS and cadmium in soil ecosystems.【Method】A laboratory incubation experiment was performed and a combined toxic effect prediction model was used to evaluate ecological toxicity of single-factor and/or combined pollution of triclosan and cadmium on soil microbial and enzyme activities involved in soil carbon and nitrogen cycling. The incubation experiment was designed to have four treatments for triclosan contamination, i.e. 0, 1.0, 10.0 and 50.0 mg kg-1 dry soil, two treatments for Cd contamination, i.e. 0 and 10.0 mg kg-1 dry soil, added in the form of Cd Cl2, three combined contamination treatments, i.e. 1.0/10.0, 10.0/10.0 and 50.0/10.0 mg kg-1 dry soil in triclosan/cadmium, and three controls containing neither cadmium nor triclosan. Each treatment had three replicates. The treated samples were incubated at 25℃ in dark. The soil respiration, protease and sucrose activities were measured on the 7 th, 14 th, 28 th and 56 th day of incubation.【Result】Results show that both the single-factor and combined pollutions of triclosan and cadmium had soil respiration activated, inhibited and then activated again. In the soils under singlefactor stress of either Cd or TCS, soil respiration was significantly activated on Day 7 with activation rate being 18.52 %, 25.26%, 27.98% and 18.45% for Cd 10.0 mg kg-1, triclosan 1.0, 10.0 and 50.0 mg kg-1, respectively, and began to be inhibited after 14 days of incubation, and activated again at the end of the incubation（Day 56）, with activation rate being 24.16%, 41.84%, 31.56% and 21.41% for Cd 10.0 mg kg-1, triclosan 1.0, 10.0 and 50.0 mg kg-1, respectively, which were significan

关 键 词：复合污染 土壤呼吸 土壤蛋白酶 土壤蔗糖酶 联合效应 

分 类 号：X131.3[环境科学与工程—环境科学]