灯盏细辛和赤芍配伍组方对大鼠脑缺血再灌注损伤的保护作用及对NF-κB通路的影响  

Effect of Combined Formula of Erigerontis Herba and Paeoniae Radix Rubra on Cerebral Ischemia Reperfusion Injury and NF-κB Pathway in Rats

作　　者：刘亭[1] 刘香香 陈亭亭 刘帆[1,2] 侯佳 孙佳[1] 王爱民 LIU Ting;LIU Xiang-xiang;CHEN Ting-ting;LIU Fan;HOU Jia;SUN Jia;WANG Ai-min(Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China;School of Pharmacy, Guizhou Medical University, Guiyang 550025, China;Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine Under Ministry of Education, Guiyang 550004, China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室,贵阳550004 [2]贵州医科大学药学院,贵阳550025 [3]民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《中国实验方剂学杂志》2018年第9期111-115,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81760699,81260636);贵州省科学技术厅人才团队项目(黔科合平台人才[2016]5613\5677);贵州省教育厅项目(黔教合协同创新字[2013]04)

摘　　要：目的：探讨灯盏细辛和赤芍配伍组方（辛芍）对大鼠中动脉阻断（MCAO）局灶脑缺血再灌注损伤的保护作用及其机制。方法：随机将SPF级SD大鼠分为假手术组、模型组、尼莫地平组（12.6 mg·kg（-1））和辛芍组（12.5 g·kg（-1））。给药组连续灌胃给药3 d,然后选用改良Zea Longa方法建立大鼠局灶脑缺血再灌注损伤模型,术后再连续给药7 d。采用Zea Longa5分制法对各组大鼠进行神经行为学判别;用2,3,5-氯化三苯基四氮唑（TTC）染色法测定脑梗死面积;用酶联免疫吸附法（ELISA）测定白细胞介素-6（IL-6）,白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的含量;并用蛋白质免疫印迹法（Western blot）检测p65和IκBα的磷酸化水平。结果：与假手术组比较,模型组出现明显神经功能缺失体征（P〈0.01）,脑梗死面积明显增加（P〈0.01）,说明大鼠局灶脑缺血再灌注损伤模型构建成功。与模型组比较,辛芍组方能明显改善脑缺血再灌注损伤模型大鼠的神经行为功能（P〈0.05）,明显降低脑梗死面积比（P〈0.05）,显著降低IL-6,IL-1β和TNF-α的水平（P〈0.01）,并显著降低p65和IκBα的磷酸化水平（P〈0.01）。结论：辛芍具有减轻大鼠脑缺血再灌注损伤的作用,其作用机制可能与抑制核转录因子-κB（NF-κB）通路激活相关。Objective：To investigate the protective effect and mechanism of combined formula of Erigerontis Herba and Paeoniae Radix Rubra（Xinshao） on cerebral ischemia reperfusion injury in rats.Method：The rats were randomly divided into four groups：sham operation group,model group,nimodipine group（12.6 mg·kg（-1）） and Xinshao group（12.5 g·kg（-1））.Rats were administrated with drugs for 3 days; then the cerebral ischemia-reperfusion model was established by improved Zea longa method.After surgery,the rats were administrated with drugs for 7 days.The neurological behavior was evaluated by Zea Longa method.triphenyltetrazolium Chloride（TTC） staining was performed to determine the area of cerebral infarction.interleukin-6（IL-6）,IL-1β and tumor necrosis factor-α（TNF-α） levels in serum were examined by enzymelinked immunosurbent assay（ELISA）.The levels of phosphorylation of p65 and IκBα were detected by Western blot.Result：Compared with sham operation group,the score of neurological behavior and the percentage of infarct area were significantly increased in model group,which indicated the successful establishment of the MACO model.Xinshao could significantly ameliorate the neurological behavior（P 〈 0.05）,and decrease the percentage of infarct area（P 〈 0.05）.Compared with model group,the levels of IL-6,IL-1β and TNF-α in serum were significantly lowered in Xinshao group（P 〈 0.01）.Western blot analysis showed that Xinshao could reduce the phosphorylation levels of p65 and IκBα（P 〈 0.01）.Conclusion：Xinshao has a protective effect on cerebral ischemia reperfusion injury in rats,which may be associated with the inhibition of nuclear transcription factor-κB（NF-κB） pathway.

关 键 词：灯盏细辛 赤芍 脑缺血再灌注 核转录因子-κB(NF-κB)通路 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]