强心康颗粒对慢性心衰大鼠心肌细胞病理形态学腺苷酸转位酶,PGC-1α mRNA表达的影响  

Effect of Qiangxinkang Granule on Pathomorphology of Cardiac Myocytes and Expressions of Adenylate Translocation Enzyme mRNA and PGC-1α mRNA in Rats with Chronic Heart Failure

作　　者：张晓华[1] 刘淑荣[1] 钱锋[1] 邸琳[1] 刘新宇[1] 赵宏宇[1] 张春柳[1] ZHANG Xiao-hua;LIU Shu-rong;QIAN Feng;DI Lin;LIU Xin-yu;ZHAO Hong-yu;ZHANG Chun-liu(Jilin Provincial Academy of Traditional Chinese Medicine, Changchun 130021, China)

机构地区：[1]吉林省中医药科学院

出　　处：《中国实验方剂学杂志》2018年第9期121-126,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林自然科学基金项目(20160101196JC)

摘　　要：目的：通过观察强心康颗粒对心衰大鼠心肌细胞病理形态学及心肌线粒体腺苷酸转位酶（ANT）,过氧化物酶体增生物激活受体γ共激活因子-1α（PGC-1α）mRNA表达的影响来探讨其治疗心衰的机制。方法：选择健康雄性SD大鼠,应用腹腔注射阿霉素（3 mg·kg（-1））,造成心衰大鼠模型,然后分为正常组,模型组,芪苈强心组（芪苈强心胶囊,0.65 g·kg（-1））,曲美他嗪组（10.8 mg·kg（-1））及强心康高、中、低剂量组（20,10,5 g·kg（-1））,造模之后进行心功能的检测,验证模型是否成立,服药4周后,苏木素-伊红（HE）及电镜扫描观察心肌细胞病理形态学变化,采用实时荧光定量聚合酶链式反应（Real-time PCR）观察7组大鼠心肌细胞病理形态学及心肌ANT,PGC-1αmRNA表达的变化。结果：与正常组比较,模型组大鼠心率明显减慢,左心室最大上升和下降速率均降低,左心室收缩压显著降低,左室终末舒张压明显增加,收缩压、舒张压、平均血压显著降低（P〈0.01）,表明心衰模型成立;模型组大鼠心肌细胞病变较明显,模型组大鼠心肌细胞病变改善明显,ANT,PGC-1αmRNA含量显著减少（P〈0.01）。与模型组比较,盐酸曲美他嗪组、芪苈强心组、强心康高、中剂量组的均能显著增加ANT,PGC-1αmRNA含量（P〈0.01）;强心康低剂量组能明显增加ANT,PGC-1αmRNA含量,与模型组比较有统计学差异（P〈0.05）。结论：强心康颗粒可通过改善心肌细胞结构,增加ANT,PGC-1αmRNA含量来纠正心功能。Objective：To study the effect of Qiangxinkang granule on the pathomorphology of cardiac myocytes and the expressions of adenylate translocation enzyme（ANT） and peroxisome proliferator activated receptor γ coactivator-1 alpha（PGC-1α） mRNA in rats with heart failure.Method：Healthy Wistar rats were selected and injected with adriamycin through abdominal cavity（3 mg·kg（-1）,once a week） for six weeks to make the model of HF rats.Then they were divided into seven groups：blank group,model group,Qili Qiangxin group（0.65 g·kg（-1））,Trimetazidine hydrochloride group（10.8 mg·kg（-1））,and high,medium and low-dose Qiangxinkang granule groups（20,10,5 g·kg（-1））.After modeling,the cardiac function was tested to verify the successful modeling.Four weeks later,htoxylin eosin（HE） staining and electron microscopy were used to observe the pathological changes of myocardial cells,and quantitative polymerase chain reaction（Real-time PCR） was used to observe the pathological changes of myocardial cells and the expressions of adenylate translocation enzyme（ANT） mRNA and PGC-1α mRNA in the seven groups.Result：Compared with the blank group,the model group showed significant slowdown in the heart rate,reduction in the maximum rising and falling rate at the left ventricle,obvious decrease in the left ventricular systolic pressure,remarkable increase in the left ventricular end-diastolic pressure and significant decrease in systolic pressure,diastolic pressure and average blood pressure（P 〈 0.01）,indicating the establishment of the heart failure model; the model group showed more significant myocardial cell lesions and more obvious alleviation in myocardial cell lesions,and decrease in ANT,PGC-1α mRNA content（P 〈 0.01）.Compared with the model group,Trimetazidine hydrochloride group,qili cardiac tonic group,and high and medium-dose Qiangxinkang granule groups showed a significant increase in ANT,PGC-1α mRNA content（P 〈 0.01）; the low-dose Qiangxinkang granule

关 键 词：慢性心力衰竭 强心康颗粒 腺苷酸转位酶 过氧化物酶体增生物激活受体γ共激活因子-1α(PGC-1α) 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]