PBNA-413对心肌缺血/再灌注损伤大鼠凋亡相关蛋白及基因表达的影响  

Protective Effect of PBNA-413 on Myocardial Ischemia Reperfusion Rats by Inhibiting Apoptosis

作　　者：汪琪[1] 廖坚 谢渭林 许培华[1] 曾丽梅[1] 黄志华[1] WANG Qi;LIAO Jian;XIE Wei-lin;XU Pei-hua;ZENG Li-mei;HUANG Zhi-hua(Gannan Medical University, Ganzhou 341000, China)

机构地区：[1]赣南医学院

出　　处：《中国实验方剂学杂志》2018年第9期134-139,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省自然科学基金项目(20122BAB205039)

摘　　要：目的：探讨拳参正丁醇提取物-413（Bistortae Rhizoma n-butyl alcohol extract,PBNA-413）对大鼠心肌缺血/再灌注损伤（myocardial ischemia/reperfusion,MI/R）细胞凋亡的影响。方法：将30只SD雄性大鼠随机分为5组,假手术组,MI/R模型组,PBNA-413低剂量组（0.1 mg·kg（-1））,PBNA-413中剂量组（0.3 mg·kg（-1））,PBNA-413高剂量组（1.0 mg·kg（-1））。采用结扎左冠状动脉前降支40 min,恢复血流60 min,复制MI/R模型,记录肢体Ⅱ导联心电图。采用实时荧光定量PCR（Real-time PCR）和蛋白免疫印迹法（Western blot）检测各组大鼠心肌组织凋亡相关蛋白B细胞淋巴瘤/白血病-2（Bcl-2）,Bcl-2相关X蛋白（Bax）,半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）,Caspase-9 mRNA及蛋白表达。结果：大鼠心肌缺血后,模型组大鼠心电图ST段及T波较假手术组明显升高（P〈0.05,P〈0.01）,PBNA-413处理后ST段及T波降低（P〈0.01）,以中剂量和高剂量的效果好。与假手术组比较,模型组大鼠心肌组织Bax,Caspase-3及Caspase-9蛋白表达升高（P〈0.05,P〈0.01）,Bcl-2蛋白表达降低（P〈0.01）;与模型组比较,PBNA-413中剂量组Bax及Caspase-3和Caspase-9蛋白表达降低（P〈0.05,P〈0.01）,Bcl-2蛋白表达升高（P〈0.01）。除Caspase-9外,Bax,Bcl-2和Caspase-3的基因水平出现与蛋白类似变化。结论：PBNA-413可能通过抑制细胞凋亡从而有效保护MI/R心肌。Objective：To investigate the effect of Bistortae Rhizoma n-butyl alcohol extract（PBNA-413）on cardiocyte apoptosis in rats with myocardial ischemia-reperfusion（MI/R） injury.Method：Thirty male Sprague-Dawley rats were randomly divided into five groups：sham group,MI/R model groups,low-dose,middledose and high-dose（0.1,0.3,1.0 mg·kg^（-1）） PBNA-413 treatment groups（n = 6）.The models of MI/R injury were established by ligating the left anterior descending coronary artery for 40 min and then reperfusing for 60 min,and the electrocardiogram（ECG） was recorded.Western blot analysis and Real-time PCR were utilized to measure B-cell lymphoma-2（Bcl-2）,Bcl-2 associated X protein（Bax）,cysteine aspartate protease-3（Caspase-3） and Caspase-9 mRNA and protein expressions in the ischemic and reperfusion region.Result：After myocardial ischemia,ECG ST-segment and T-wave in MI/R vehicle group were significantly higher than those in sham group（P 〈 0.05,P 〈 0.01）,but they were significantly decreased in the PBNA-413 treatment groups（P 〈 0.01）,particularly in middle and high-dose groups.Compared with Sham group,protein and mRNA expressions of Bcl-2（P 〈 0.01） were decreased,while Bax,Caspase-3 and Caspase-9 were increased in MI/R vehicle group（P 0.05,P 〈 0.01）.Compared with MI/R vehicle group,the middle-dose PBNA-413 group showed an increased protein expression of Bcl-2（P 〈 0.05,P 〈 0.01）,but decreased expressions of Bax,Caspase-3 and Caspase-9（P 〈 0.05,P 〈 0.01）.Except for Caspase-9,mRNA expressions of Bax,Bcl-2,and Caspase-3 showed similar changes with protein expressions.Conclusion：PBNA-413 may play a protective role against MI/R injury in rats by inhibiting cell apoptosis.

关 键 词：拳参正丁醇提取物-413(PBNA-413) 心肌缺血/再灌注 细胞凋亡 B细胞淋巴瘤/白血病-2(Bcl-2) Bcl-2相关X蛋白(Bax) 半胱氨酸天冬氨酸蛋白酶(Caspase)-3 CASPASE-9 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]